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Simulations of DNA-Origami Self-Assembly Reveal Design-Dependent Nucleation Barriers.
| Content Provider | Europe PMC |
|---|---|
| Author | Cumberworth, Alexander Frenkel, Daan Reinhardt, Aleks |
| Copyright Year | 2022 |
| Abstract | Nucleation is the rate-determining step in the kineticsof manyself-assembly processes. However, the importance of nucleation inthe kinetics of DNA-origami self-assembly, which involves both thebinding of staple strands and the folding of the scaffold strand,is unclear. Here, using Monte Carlo simulations of a lattice modelof DNA origami, we find that some, but not all, designs can have anucleation barrier and that this barrier disappears at lower temperatures,rationalizing the success of isothermal assembly. We show that theheight of the nucleation barrier depends primarily on the coaxialstacking of staples that are adjacent on the same helix, a parameterthat can be modified with staple design. Creating a nucleation barrierto DNA-origami assembly could be useful in optimizing assembly timesand yields, while eliminating the barrier may allow for fast molecularsensors that can assemble/disassemble without hysteresis in responseto changes in the environment. |
| ISSN | 15306984 |
| Journal | Nano Letters |
| Volume Number | 22 |
| PubMed Central reference number | PMC9479157 |
| Issue Number | 17 |
| PubMed reference number | 36037484 |
| e-ISSN | 15306992 |
| DOI | 10.1021/acs.nanolett.2c01372 |
| Language | English |
| Publisher | American Chemical Society |
| Publisher Date | 2022-08-29 |
| Access Restriction | Open |
| Rights License | Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2022 The Authors. Published by American Chemical Society |
| Subject Keyword | DNA origami self-assembly control of nucleation isothermal assembly coarse-grained models |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Chemistry Condensed Matter Physics Bioengineering Materials Science Mechanical Engineering |
