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Back cover

Molecular BioSystems Vol - 9 Issue - 5

Molecular BioSystems Vol - 9 Issue - 4

Molecular BioSystems Vol - 9 Issue - 3

Molecular BioSystems Vol - 9 Issue - 2

Molecular BioSystems Vol -8

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Rapid mass spectrometric determination of disulfide connectivity in peptides and proteins

Content Provider	Royal Society of Chemistry (RSC)
Author	Balaram, Padmanabhan Gowd, Konkallu Hanumae Bhattacharyya, Moitrayee Gupta, Kallol
Copyright Year	2013
Abstract	Disulfide crosslinks are ubiquitous in natural peptides and proteins, providing rigidity to polypeptide scaffolds. The assignment of disulfide connectivity in multiple crosslinked systems is often difficult to achieve. Here, we show that rapid unambiguous characterisation of disulfide connectivity can be achieved through direct mass spectrometric CID fragmentation of the disulfide intact polypeptides. The method requires a direct mass spectrometric fragmentation of the native disulfide bonded polypeptides and subsequent analysis using a newly developed program, DisConnect. Technical difficulties involving direct fragmentation of proteins are surmounted by an initial proteolytic nick and subsequent determination of the structures of these proteolytic peptides through DisConnect. While the connectivity in proteolytic fragments containing one cystine is evident from the MS profile alone, those with multiple cystines are subjected to subsequent mass spectrometric fragmentation. The wide applicability of this method is illustrated using examples of peptide hormones, peptide toxins, proteins, and disulfide foldamers of a synthetic analogue of a marine peptide toxin. The method, coupled with DisConnect, provides an unambiguous, straightforward approach, especially useful for the rapid screening of the disulfide crosslink fidelity in recombinant proteins, determination of disulfide linkages in natural peptide toxins and characterization of folding intermediates encountered in oxidative folding pathways.
Starting Page	1340
Ending Page	1350
Page Count	11
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	6
Journal	Molecular BioSystems
DOI	10.1039/c3mb25534d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Disulfide The Assignment (1997 film) Peptide Criminal investigation department Cystine
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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