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Molecular BioSystems Vol - 9 Issue - 5

Molecular BioSystems Vol - 9 Issue - 4

Molecular BioSystems Vol - 9 Issue - 3

Molecular BioSystems Vol - 9 Issue - 2

Molecular BioSystems Vol -8

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Red blood cell metabolism under prolonged anaerobic storage

Content Provider	Royal Society of Chemistry (RSC)
Author	Zolla, Lello D'Alessandro, Angelo Gevi, Federica
Copyright Year	2013
Abstract	Oxygen dependent modulation of red blood cell metabolism is a long investigated issue. However, the recent introduction of novel mass spectrometry-based approaches lends itself to implement our understanding of the effects of red blood cell prolonged exposure to anaerobiosis. Indeed, most of the studies conducted so far have addressed the short term issue, while the limited body of literature covering a 42 days storage period only takes into account a handful of metabolic parameters (ATP, DPG, glucose, glyceraldehyde 3-phosphate, and lactate). We hereby performed a mass spectrometry-based untargeted metabolomics analysis in order to highlight metabolic species in erythrocyte concentrates stored anaerobically in SAGM additive solutions for up to 42 days, by testing cells on a weekly basis. We could confirm previous evidence about long term anaerobiosis promoting glycolytic metabolism in RBCs and prolonging the conservation of high energy phosphate reservoirs and purine homeostasis. In parallel, we evidenced that, in contrast to aerobic storage, anaerobiosis impairs erythrocyte capacity to cope with oxidative stress by blocking metabolic diversion towards the pentose phosphate pathway, which negatively affects glutathione homeostasis. Therefore, although oxidative stress was less sustained than in aerobically stored counterparts, oxidative stress markers still accumulate over anaerobic storage progression.
Starting Page	1196
Ending Page	1209
Page Count	14
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	6
Journal	Molecular BioSystems
DOI	10.1039/c3mb25575a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Metabolomics Oxidative stress Anaerobic respiration Red blood cell Homeostasis Glucose Glyceraldehyde 3-phosphate Metabolism Phosphate Pentose Cellular respiration DPG Purine Lactic acid ATP Adenosine triphosphate Glutathione
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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