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Molecular BioSystems Vol - 9 Issue - 5

Molecular BioSystems Vol - 9 Issue - 4

Molecular BioSystems Vol - 9 Issue - 3

Molecular BioSystems Vol - 9 Issue - 2

Molecular BioSystems Vol -8

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Complementary biochemical approaches applied to the identification of plastidial calmodulin-binding proteins

Content Provider	Royal Society of Chemistry (RSC)
Author	Giustini, Cécile Ferro, Myriam Curien, Gilles Salvi, Daniel Baudet, Mathieu Seigneurin-Berny, Daphné Dell'Aglio, Elisa Delpierre, Faustine Moyet, Lucas Rolland, Norbert Brugière, Sabine Matringe, Michel
Copyright Year	2013
Abstract	Ca2+/Calmodulin (CaM)-dependent signaling pathways play a major role in the modulation of cell responses in eukaryotes. In the chloroplast, few proteins such as the NAD+ kinase 2 have been previously shown to interact with CaM, but a general picture of the role of Ca2+/CaM signaling in this organelle is still lacking. Using CaM-affinity chromatography and mass spectrometry, we identified 210 candidate CaM-binding proteins from different Arabidopsis and spinach chloroplast sub-fractions. A subset of these proteins was validated by an optimized in vitro CaM-binding assay. In addition, we designed two fluorescence anisotropy assays to quantitatively characterize the binding parameters and applied those assays to NAD+ kinase 2 and selected candidate proteins. On the basis of our results, there might be many more plastidial CaM-binding proteins than previously estimated. In addition, we showed that an array of complementary biochemical techniques is necessary in order to characterize the mode of interaction of candidate proteins with CaM.
Starting Page	1234
Ending Page	1248
Page Count	15
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	6
Journal	Molecular BioSystems
DOI	10.1039/c3mb00004d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Arabidopsis thaliana Anisotropy Gene regulatory network Organelle Fluorescence microscope Spinach Mass spectrometry Calmodulin Chloroplast Chromatography
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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Complementary biochemical approaches applied to the identification of plastidial calmodulin-binding proteins

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