NDLI: Proteomic and ionomic profiling reveals significant alterations of protein expression and calcium homeostasis in cystic fibrosis cells

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Molecular BioSystems Vol - 9 Issue - 5

Molecular BioSystems Vol - 9 Issue - 4

Molecular BioSystems Vol - 9 Issue - 3

Molecular BioSystems Vol - 9 Issue - 2

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Molecular BioSystems Vol -2

Proteomic and ionomic profiling reveals significant alterations of protein expression and calcium homeostasis in cystic fibrosis cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Consalvo, Ada Ciavardelli, Domenico Sacchetta, Paolo D'Orazio, Melania Ilio, Carmine Di Battistoni, Andrea Rossi, Claudia Urbani, Andrea Pieroni, Luisa
Copyright Year	2013
Abstract	Cystic fibrosis (CF) is an autosomal recessive disorder associated with mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene and defective chloride transport across the epithelial cell membranes. Abnormal epithelial ion transport is the primary cause of persistent airway infections and chronic inflammation in CF patients. In order to gain further insight into the mechanisms of epithelial dysfunctions linked to CFTR mutations, we performed and integrated proteomic and ionomic analysis of human bronchial epithelial IB3-1 cells and compared them with a CFTR-complemented isogenic cell line (C38). Aside from changes that were consistent with known effects related to CFTR mutations, such as differences in glycolytic and gluconeogenic pathways and unfolded protein responses, differential proteomics highlighted significant alteration of protein expression and, in particular, of the 14-3-3 signalling pathway that is known to be involved in cellular calcium (Ca) homeostasis. Of note, restoring chloride efflux by acting on Ca cellular homeostasis has been shown to be a promising therapeutic intervention for CF. Ionomic analysis showed significant changes in the IB3-1 element profile compared with C38 cells and in particular we observed an increase of intracellular Ca that significantly correlates with intracellular zinc (Zn) levels, suggesting a synergistic role of Ca and Zn influx. This finding is particularly intriguing because Zn has been reported to be effective in CF treatment increasing Ca influx. Taken together, our proteomic and ionomic data reveal that CFTR mutation sets in motion endogenous mechanisms counteracting impaired chloride transport mainly acting on epithelial ion transport and increasing intracellular Ca, suggesting potential links between protein expression and this response.
Starting Page	1117
Ending Page	1126
Page Count	10
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	9
Issue Number	6
Journal	Molecular BioSystems
DOI	10.1039/c3mb25594h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Calcium IB3 (TV channel) Homeostasis Ion Inflammation Advertisement film Epithelium Protein Zinc Chloride Gene Fibrosis Proteomics Mutation Cystic fibrosis transmembrane conductance regulator CFTR Dominance (genetics)
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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