NDLI: Highly sensitive aptasensor for oxytetracycline based on upconversion and magnetic nanoparticles

Content Provider	Royal Society of Chemistry (RSC)
Author	Fang, Congcong Wang, Zhouping Duan, Nuo Wu, Shijia Dai, Shaoliang
Copyright Year	2015
Abstract	A novel sensitive aptasensor was developed for the quantification of oxytetracycline (OTC) in this study. Artificial aptamer-modified magnetic nanoparticles (aptamer-MNPs) were employed as capture probes, and complementary oligonucleotide-modified upconversion nanoparticles (cDNA-UCNPs) were used as signal probes. Then, the probes were hybridized to form poly-network structure MNPs-UCNPs signal probes. Finally, when the target was introduced, the aptamer combined with the priority target and the signal probe was replaced. The proposed method achieved a linear range between 0.05 and 100 ng mL−1, and the limit of detection (LOD) was as low as 0.036 ng mL−1, benefiting largely from labeling with UCNPs, aptamer affinity and magnetic separation. Then, we successfully applied the method to measure OTC in milk samples and validated it by a commercially available enzyme-linked immunosorbent assay (ELISA) method. The results demonstrated that the method possessed high sensitivity and good selectivity for the determination of OTC and is applicable to the determination of OTC in food samples.
Starting Page	2585
Ending Page	2593
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c4ay03035d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oxytetracycline Aptamer ELISA
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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