NDLI: A Raman peak recognition method based automated fluorescence subtraction algorithm for retrieval of Raman spectra of highly fluorescent samples

Content Provider	Royal Society of Chemistry (RSC)
Author	Wu, Tao Zhang, Hongyuan Li, Yan Chen, Kun Wei, Haoyun
Copyright Year	2015
Abstract	Intense fluorescence background is a major problem in the application of Raman spectroscopy. An appropriate algorithm which can faithfully retrieve weak tissue Raman signals is required. In this article, we propose a new algorithm for automated and artifact-free recovery of Raman spectra which combines a novel Raman peak recognition method (RPR method) with an improved iterative smoothing method (SG-SR method). The SG-SR method, based on the modified Savitzky–Golay iterative process, substantially improves its convergence speed. By applying a novel negative relaxation factor to the successive relaxation iterative method, automatic recognition of Raman peaks is realized. In the proposed algorithm (RIA-SG-RPR algorithm), a real Raman peak position is first detected by the RPR method to serve as the intrinsic criterion of convergence for the SG-SR method to avoid human interference. Then, real Raman signals are recovered from the iterative procedure of the SG-SR method. This algorithm has been optimized and validated with mathematically simulated Raman spectra as well as experimentally recorded Raman spectra from various fluorescent samples, resulting in a significant improvement in the rejection of both high fluorescence background and direct human intervention. This algorithm drastically avoids false Raman features to benefit the utilization of Raman spectroscopy to characterize molecular specifics in more challenging Raman applications.
Starting Page	2770
Ending Page	2778
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c4ay03025g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Golay Rapid plasma reagin RPR Fluorescence Iterative method Raman Raman spectroscopy Savitzky Algorithm Flat module
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A Raman peak recognition method based automated fluorescence subtraction algorithm for retrieval of Raman spectra of highly fluorescent samples

Improved Savitzky–Golay-method-based fluorescence subtraction algorithm for rapid recovery of Raman spectra

Comparison of Quantitative Results among Two Automated Rapid Plasma Reagin (RPR) Assays and a Manual RPR Test

Why and How Savitzky-Golay Filters Should Be Replaced.

Comparison of an automated rapid plasma reagin (RPR) test with the conventional RPR card test in syphilis testing.

Rapid plasma reagin ( RPR ) card test

Octane Number Detection Based on Raman Spectra

Optimization of Savitzky-Golay smoothing filter for salivary surface enhanced Raman spectra of non structural protein 1

Comparison of an automated rapid plasma reagin (RPR) test with the conventional RPR card test in syphilis testing

A Raman peak recognition method based automated fluorescence subtraction algorithm for retrieval of Raman spectra of highly fluorescent samples

Similar Documents

A Raman peak recognition method based automated fluorescence subtraction algorithm for retrieval of Raman spectra of highly fluorescent samples

Improved Savitzky–Golay-method-based fluorescence subtraction algorithm for rapid recovery of Raman spectra

Comparison of Quantitative Results among Two Automated Rapid Plasma Reagin (RPR) Assays and a Manual RPR Test

Why and How Savitzky-Golay Filters Should Be Replaced.

Comparison of an automated rapid plasma reagin (RPR) test with the conventional RPR card test in syphilis testing.

Rapid plasma reagin ( RPR ) card test

Octane Number Detection Based on Raman Spectra

Optimization of Savitzky-Golay smoothing filter for salivary surface enhanced Raman spectra of non structural protein 1

Comparison of an automated rapid plasma reagin (RPR) test with the conventional RPR card test in syphilis testing

A Raman peak recognition method based automated fluorescence subtraction algorithm for retrieval of Raman spectra of highly fluorescent samples