NDLI: Uncertainty in CCD detectors with and without cooling devices when used for molecular fluorescence measurements

Content Provider	Royal Society of Chemistry (RSC)
Author	Ostra, Miren Galbán, Javier Ubide, Carlos Aramburu, Itxaso Zuriarrain, Juan Vidal, Maider
Copyright Year	2015
Abstract	The performance of CCD detectors for fluorescence measurements is evaluated through uncertainty studies, mainly as a function of both the signal intensity and the detector temperature. Two CCD detectors, one furnished with a cooling device and the other without a cooling device, were used, and the results were compared. The dependence of uncertainty on the instrumental signal was evaluated simultaneously for both detectors at temperatures ranging between −23 and 23 °C. The tested detectors required temperature increments between 30 and 50 °C to double dark noise (the random part of dark current). The detector temperature affects uncertainty, but this is basically related to dark noise in fluorescence measurements. The consequence of this is that the temperature does not significantly affect the signal-to-noise ratio (S/N); thus, the presence of a cooling device does not significantly improvement the performance, and the limit of detection (LOD) does not depend strongly on the detector temperature. The quality of the two-dimensional array does affect uncertainty, the value of S/N, and the LOD. A good CCD detector can perform at a level similar to a spectrofluorometer furnished with a photomultiplier tube. Laboratory data are given to show how the three components of uncertainty (dark noise, shot noise and flicker noise) behave at different signal intensities and temperatures. Dark noise is the most important factor, shot noise has relevance only at high signal values, and flicker noise is practically irrelevant. The classical model of the dependence of S/N on the fluorescence signal is applied, and the uncertainty constants that govern the performance of the apparatus used are given.
Starting Page	2379
Ending Page	2385
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c4ay02934h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	The Tested Charge-coupled device Spectrofluorometer Classical physics Dependent and independent variables Fluorescence Signal-to-noise ratio Photomultiplier Photoreceptor cell Shot noise Flicker noise
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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