NDLI: Application of titanium dioxide nanowires and electroreduced graphene oxide modified electrodes for the electrochemical detection of specific <i>tlh</i> gene sequence from <i>Vibrio parahaemolyticus</i>

Content Provider	Royal Society of Chemistry (RSC)
Author	Sun, Wei Wu, Fan Li, Guangjiu Cheng, Yong Liu, Lihua Wu, Simeng Wang, Xiuli Zheng, Wen
Copyright Year	2015
Abstract	In this paper, an electrochemical DNA biosensor was prepared using electroreduced graphene oxide, titanium dioxide nanowires and chitosan modified carbon ionic liquid electrode as the substrate electrode, which was further used for the electrochemical detection of the specific tlh gene sequence from Vibrio parahaemolyticus. The presence of nanocomposite on the electrode surface can increase the surface area and fix the ssDNA probe sequence by electrostatic attraction. After the hybridization with the target ssDNA sequence under the selected conditions, methylene blue (MB) was used as the electrochemical indicator for monitoring the hybridization reaction. Under the optimal conditions, the reduction peak current of MB was proportional to the concentration of tlh gene sequence in the range from 1.0 × 10−12 to 1.0 × 10−6 mol L−1 with a detection limit as 3.17 × 10−13 mol L−1 (3σ). This electrochemical DNA sensor exhibited good ability to discriminate between one-base and three-base mismatched ssDNA sequences, and the polymerase chain reaction amplification product of the tlh gene from oyster samples was further detected with satisfactory results.
Starting Page	2623
Ending Page	2629
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c4ay02932a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Chain reaction Electrode Carbon Ionic liquid Methylene blue Gene Graphene DNA Biosensor Electrochemistry Nanocomposite Chitosan Titanium dioxide Vibrio
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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