NDLI: Spectrophotometric determination of cyclamate in artificial sweeteners and beverages after ultrasound-assisted emulsification microextraction

Content Provider	Royal Society of Chemistry (RSC)
Author	Zohrabi, Parvin Abdolhosseini, Sana Hashemi, Mahdi
Copyright Year	2015
Abstract	A new method has been developed for the determination of cyclamate using an ultrasound-assisted emulsification microextraction (USAE-ME) procedure coupled with UV-Vis spectrophotometry. The method is based on the protonation of cyclamate ions in acidic medium and the extraction of the formed cyclamic acid into fine droplets of chloroform as an extraction solvent that contains rhodamine B (RhB) reagent. The extracted cyclamic acid can further react with RhB for the formation of a highly colored ion-pair complex of [cyclamate][RhBH+], which is used for the subsequent spectrophotometric determination of cyclamate. One variable at a time optimization and response surface methodology (RSM) based on central composite design were used to obtain optimum conditions for the microextraction procedure, and nearly the same experimental conditions were obtained using both the optimization methods. Under the optimum conditions the calibration graph was linear over the range of 50–900 ng mL−1 (R2 = 0.9994), and the limit of detection (S/N = 3) was estimated to be 10 ng mL−1. The relative standard deviation for 200 ng mL−1 of cyclamate was 2.3% (n = 5). The proposed method was successfully applied for the determination of cyclamate in beverages and sweetener tablets. The average recovery of spiked samples was 99.7%. The results demonstrated that the developed method is simple, rapid, inexpensive, accurate and remarkably free from interference effects.
Starting Page	2594
Ending Page	2602
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c4ay02903h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Rhodamine Acid Central composite design Chloroform Response surface methodology Reagent Coefficient of variation Graph of a function Sodium cyclamate Emulsion Spectrophotometry Solvent Mathematical optimization
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Novel ultrasound-assisted mixed anionic–cationic surfactant-enhanced emulsification microextraction combined with HPLC for the determination of carbamate pesticides

Response surface methodology optimized dispersive liquid–liquid microextraction coupled with UV-Vis spectrophotometry for determination of quinine

Trace determination of linear alkylbenzene sulfonates using ionic liquid based ultrasound-assisted dispersive liquid–liquid microextraction and response surface methodology

A simplified vortex-assisted emulsification microextraction method for determining personal care products in environmental water samples by ultra-high-performance liquid chromatography

Rapid analysis of fungicides in white wines from Northwest Spain by ultrasound-assisted emulsification-microextraction and gas chromatography-mass spectrometry

Ultrasound Assisted Surfactant Enhanced Emulsification Microextraction and Spectrofluorimetry for Determination of Oxadiazon in Agricultural Water Samples

Ultrasound assisted emulsification microextraction for selective determination of trace amount of mercury(II)

Anionic surfactant coacervation extraction-magnetic solid phase microextraction for determination of malachite green

Ultrasound-assisted Emulsification Microextraction-solvent Floating Solidification- Pre-column Derivatization for the Determination of Estrogens in Water Samples

Spectrophotometric determination of cyclamate in artificial sweeteners and beverages after ultrasound-assisted emulsification microextraction

Similar Documents

Novel ultrasound-assisted mixed anionic–cationic surfactant-enhanced emulsification microextraction combined with HPLC for the determination of carbamate pesticides

Response surface methodology optimized dispersive liquid–liquid microextraction coupled with UV-Vis spectrophotometry for determination of quinine

Trace determination of linear alkylbenzene sulfonates using ionic liquid based ultrasound-assisted dispersive liquid–liquid microextraction and response surface methodology

A simplified vortex-assisted emulsification microextraction method for determining personal care products in environmental water samples by ultra-high-performance liquid chromatography

Rapid analysis of fungicides in white wines from Northwest Spain by ultrasound-assisted emulsification-microextraction and gas chromatography-mass spectrometry

Ultrasound Assisted Surfactant Enhanced Emulsification Microextraction and Spectrofluorimetry for Determination of Oxadiazon in Agricultural Water Samples

Ultrasound assisted emulsification microextraction for selective determination of trace amount of mercury(II)

Anionic surfactant coacervation extraction-magnetic solid phase microextraction for determination of malachite green

Ultrasound-assisted Emulsification Microextraction-solvent Floating Solidification- Pre-column Derivatization for the Determination of Estrogens in Water Samples

Spectrophotometric determination of cyclamate in artificial sweeteners and beverages after ultrasound-assisted emulsification microextraction