NDLI: Dairy manure protein analysis using UV-vis based on the Bradford method

Content Provider	Royal Society of Chemistry (RSC)
Author	Liu, Zong Wang, Hui Gonzalez, Jorge Santini Gunasekaran, Sundaram Runge, Troy
Copyright Year	2015
Abstract	Organic nitrogen in the form of protein and degraded protein fragments is one of the most important components of dairy manure. Conventionally, protein concentration in dairy manure was estimated by crude protein but it is often overestimated because crude protein contains non-protein nitrogen (NPN). The Bradford assay is predominantly used in measuring clinic samples. In this paper, dairy manure protein assays including the Bradford assay, total Kjeldahl nitrogen method and amino acid analysis were compared using various types of manure samples with a wide range of solids contents at different on-farm treatment steps. The manure protein concentration assay by UV-vis using Bradford method was validated to be compatible to measure the protein concentration in manure samples. Relationships for estimating manure total protein concentration from soluble protein based on the solids contents have been determined. The method described in this study could be used as a supplement method for traditional protein assay, especially when the solids content in manure samples is low. In addition, this method can be easily adapted at a farm level to monitor manure soluble protein and estimate crude protein concentration in order to evaluate the effects of diet change and to manage manure field application amount as fertilizer.
Starting Page	2645
Ending Page	2652
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	7
Issue Number	6
Journal	Analytical Methods
DOI	10.1039/c4ay03006k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Non-protein nitrogen Acid Bradford Bradford protein assay Kjeldahl method Nitrogen NPN Protein Fertilizer
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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LINEARIZATION OF THE BRADFORD PROTEIN ASSAY TO APPLICATION IN COW MILK PROTEINS QUANTIFICATION BY UV-Vis SPECTROPHOTOMETRY METHOD

DETERMINATION OF NITROGEN IN BORON NITRIDE.

Comparison of kjeldahl and dumas methods for determining protein contents of soybean products

A method for determining total nitrogen in Kjeldahl digestion solution using a centrifugal analyser.

Protein Determination—Method Matters

A method for determining total nitrogen in Kjeldahl digestion solution using a centrifugal analyser

The early years of the kjeldahl method to determine nitrogen*.

Quantitation of nucleoprotein complexes by UV absorbance and Bradford assay.

Food composition and additives kjeldahl nitrogen analysis as a reference method for protein determination in dairy products.

Dairy manure protein analysis using UV-vis based on the Bradford method

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LINEARIZATION OF THE BRADFORD PROTEIN ASSAY TO APPLICATION IN COW MILK PROTEINS QUANTIFICATION BY UV-Vis SPECTROPHOTOMETRY METHOD

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Comparison of kjeldahl and dumas methods for determining protein contents of soybean products

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A method for determining total nitrogen in Kjeldahl digestion solution using a centrifugal analyser

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Dairy manure protein analysis using UV-vis based on the Bradford method