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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Chen, Xiaomin Ma, Jianshe Xiang, Zheng Zhang, Shuangshuang Shi, Shaohua Hu, Shuping |
| Copyright Year | 2014 |
| Abstract | In this work, we established a simple, sensitive and rapid method for quantitative detection of dendrobine levels in plasma using ultra fast liquid chromatography with tandem mass spectrometry (UFLC-MS/MS). The plasma samples were performed by protein precipitation and then the supernatant was extracted by chloroform. An aliquot of extracted residues was injected into UFLC-MS/MS system with separation performed by an ACQUITY HSS T3 column. Acetonitrile and water containing 10 mM ammonium acetate was used as a mobile phase. The average extraction recoveries were greater than 80.5% for dendrobine and 90.9% for matrine (Internal Standards, IS) from plasma. Good linearity (R2 > 0.992) was observed in the range of 1–1000 ng mL−1. For rat plasma, the overall accuracy of this method was 94.3–110.3%. Intra-assay and inter-assay precision was less than 11.7%. The lower limit of quantification and detection for dendrobine was 1.0 and 0.4 ng mL−1, respectively. The practical utility of this new UFLC-MS/MS method was confirmed by pharmacokinetic studies followed by sublingual intravenous administration of three levels of dendrobine in rats. |
| Starting Page | 1197 |
| Ending Page | 1202 |
| Page Count | 6 |
| File Format | HTM / HTML PDF |
| ISSN | 17599660 |
| Volume Number | 6 |
| Issue Number | 4 |
| Journal | Analytical Methods |
| DOI | 10.1039/c3ay41743c |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Protein precipitation Matrine Chloroform Dendrobine Acetonitrile Sublingual administration Pharmacokinetics Mass spectrometry Chromatography Blood plasma |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Engineering Chemical Engineering |
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