NDLI: Assessment of paraffin removal from prostate FFPE sections using transmission mode FTIR-FPA imaging

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Analytical Methods Vol - 6 Issue - 3

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Analytical Methods Vol -1

Assessment of paraffin removal from prostate FFPE sections using transmission mode FTIR-FPA imaging

Content Provider	Royal Society of Chemistry (RSC)
Author	Gaunt, Lydia Brown, Michael Gardner, Peter Hughes, Caryn Clarke, Noel W.
Copyright Year	2014
Abstract	Formalin-fixed paraffin-embedded (FFPE) tissue sections are routinely analysed for biochemical discrimination by vibrational spectroscopy techniques in the spectral pathology community. In these experiments, it is usually desirable to remove the paraffin from the tissue. This is most commonly performed by the use of xylene but other solvents such as hexane are also used. It is thought that the removal of unbound paraffin wax by such solvents also leaches out lipids native to the tissue, leading to the perception by some that any subsequent analysis on remaining lipids of dewaxed tissue may be unreliable. The scope of the study was to make an assessment of whether a dewaxing protocol can demonstrate that paraffin wax is reliably removed in relation to the detectable limits of transmission mode infrared spectroscopy. In this study, a specific sampling region of two serial FFPE sections of human prostate cancer were analysed by monitoring the methylene hydrocarbon-associated vibrations as a function of solvent immersion time during washes of either hexane or xylene. Results of the study indicate that after 5–10 minutes of immersion in the solvent the hydrocarbon-associated vibrations remain fairly consistent across the tissue. This suggests that while methylene chains of free, unbound tissue lipids are leached from the tissue (assumingly in the first tissue processing stages prior to paraffin embedding) solvent-resistant lipids remain present in formalin-fixed tissue due to being locked into protein–lipid complex matrices, predominantly in the membranes. It is these lipids signals that are subsequently detectable by spectral pathology and may still prove to be of diagnostic use.
Starting Page	1028
Ending Page	1035
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	4
Journal	Analytical Methods
DOI	10.1039/c3ay41308j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Infrared spectroscopy Pathology Methylene bridge Xylene Hexane Prostate cancer Solvent Paraffin wax
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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