NDLI: Development and validation of an UHPLC-MS/MS method for the simultaneous determination of four flavonoid glycosides and phellopterin in rat plasma: application to a pharmacokinetic study of oral administration of <i>Poncirus trifoliata</i> extract

Content Provider	Royal Society of Chemistry (RSC)
Author	Wang, Zhe Qin, Baoli
Copyright Year	2016
Abstract	A simple, sensitive and selective ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination and pharmacokinetic study of five active components, hesperidin, neohesperidin, hesperidin methyl chalcone, poncirin, and phellopterin in rat plasma after oral administration of Poncirus trifoliata extract for the first time. This method involves a simple protein precipitation extraction with acetonitrile. The separation was performed on an Agilent XDB C18 column (100 × 2.1 mm and 1.8 μm) by gradient elution using a mobile phase composed of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.35 mL min−1. Selective reaction monitoring was used for the quantification of the five active components and an internal standard (IS, oroxin B). The method was linear for all analytes over their investigated ranges with all correlation coefficients greater than 0.995. The lower limits of quantification (LLOQ) were 2.0 ng mL−1 for hesperidin, neohesperidin, hesperidin methyl chalcone, and poncirin, and 1.0 ng mL−1 for phellopterin. Intra- and inter-day precisions (RSD%) were in the range of 0.8–12.9% and the accuracies were between 87.2 and 108.3%. The developed method was successfully applied to the pharmacokinetic study of hesperidin, neohesperidin, hesperidin methyl chalcone, poncirin, and phellopterin in rat plasma after oral administration of Poncirus trifoliata extract.
Starting Page	8443
Ending Page	8451
Page Count	9
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	8
Issue Number	48
Journal	Analytical Methods
DOI	10.1039/c6ay02446g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Chalconoid Trifoliate orange Acid Protein precipitation Acetonitrile Micrometre Pharmacokinetics Neohesperidin Mass spectrometry Hesperidin Blood plasma
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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