NDLI: The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases

Content Provider	PubMed Central
Author	Bei, Lv Dai, Yunjia Liu, Ju Zhuge, Qiang Li, Dawei
Copyright Year	2015
Abstract	The effects of dimethyl sulfoxide (DMSO) on supercoiled plasmid DNA relaxation catalyzed by two typical type I topoisomerases were investigated in our studies. It is shown that DMSO in a low concentration (less than 20%, v/v) can induce a dose-related enhancement of the relaxation efficiency of Escherichia coli topoisomerase I (type IA). Conversely, obvious inhibitory effect on the activity of calf thymus topoisomerase I (type IB) was observed when the same concentration of DMSO is used. In addition, our studies demonstrate that 20% DMSO has an ability to reduce the inhibitory effect on EcTopo I, which was induced by double-stranded oligodeoxyribonucleotides while the same effect cannot be found in the case of CtTopo I. Moreover, our AFM examinations suggested that DMSO can change the conformation of negatively supercoiled plasmid by creating some locally loose regions in DNA molecules. Combining all the lines of evidence, we proposed that DMSO enhanced EcTopo I relaxation activity by (1) increasing the single-stranded DNA regions for the activities of EcTopo I in the early and middle stages of the reaction and (2) preventing the formation of double-stranded DNA-enzyme complex in the later stage, which can elevate the effective concentration of the topoisomerase in the reaction solution.
Related Links	http://dx.doi.org/10.1155/2015/320490
Starting Page	320490
File Format	PDF
ISSN	23146133
e-ISSN	23146141
Journal	BioMed Research International
Volume Number	2015
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2015-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases.

Relaxation of supercoiled phosphorothioate DNA by mammalian topoisomerases is inhibited in a base-specific manner.

Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement

Relaxation of supercoiled plasmid DNA by oxidative stresses in Escherichia coli.

Unlinking of Supercoiled DNA Catenanes by Type IIA Topoisomerases

Functional interactions of DNA topoisomerases with a human replication origin

Kinetic Pathways of Topology Simplification by Type-II Topoisomerases in Knotted Supercoiled DNA

Formation of nucleosomes on positively supercoiled DNA.

DNA damage recognition and repair by 3-methyladenine DNA glycosylase I (TAG)

The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases

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The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases.

Relaxation of supercoiled phosphorothioate DNA by mammalian topoisomerases is inhibited in a base-specific manner.

Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement

Relaxation of supercoiled plasmid DNA by oxidative stresses in Escherichia coli.

Unlinking of Supercoiled DNA Catenanes by Type IIA Topoisomerases

Functional interactions of DNA topoisomerases with a human replication origin

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The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases