NDLI: Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity

Content Provider	PubMed Central
Author	Thiel, Cora S. Hauschild, Swantje Tauber, Svantje Paulsen, Katrin Christiane, Raig Arnold, Raem Biskup, Josefine Annett, Gutewort Hürlimann, Eva Unverdorben, Felix Isabell, Buttron Lauber, Beatrice Philpot, Claudia Lier, Hartwin Engelmann, Frank Layer, Liliana E. Ullrich, Oliver
Copyright Year	2015
Abstract	Gene expression studies are indispensable for investigation and elucidation of molecular mechanisms. For the process of normalization, reference genes (“housekeeping genes”) are essential to verify gene expression analysis. Thus, it is assumed that these reference genes demonstrate similar expression levels over all experimental conditions. However, common recommendations about reference genes were established during 1 g conditions and therefore their applicability in studies with altered gravity has not been demonstrated yet. The microarray technology is frequently used to generate expression profiles under defined conditions and to determine the relative difference in expression levels between two or more different states. In our study, we searched for potential reference genes with stable expression during different gravitational conditions (microgravity, normogravity, and hypergravity) which are additionally not altered in different hardware systems. We were able to identify eight genes (ALB, B4GALT6, GAPDH, HMBS, YWHAZ, ABCA5, ABCA9, and ABCC1) which demonstrated no altered gene expression levels in all tested conditions and therefore represent good candidates for the standardization of gene expression studies in altered gravity.
Related Links	http://dx.doi.org/10.1155/2015/363575
Starting Page	363575
File Format	PDF
ISSN	23146141
e-ISSN	23146141
Journal	BioMed Research International
Volume Number	2015
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2015-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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