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The Journal of Engineering

Effect of PEI surface modification with PEG on cytotoxicity and transfection efficiency

Content Provider	IET Digital Library
Author	Saqafi, Batoul Rahbarizadeh, Fatemeh
Abstract	Polyethylenimine (PEI) is a cationic polymer with high transfection efficiency as non-viral gene delivery agent that exhibits promising features for gene therapy applications due to its cationic charge and thus favourable DNA-condensing abilities; however, its high cytotoxicity restricts its application. The cellular toxicity of PEI molecules depends on their structure, molecular weight and surface charge density. To improve the properties of branched 25 kDa PEI as a non-viral gene delivery agent, this polymer was conjugated to polyethylene glycol (PEG) molecules at three different molar ratios of 10, 20 and 30. The degree of PEG grafting was determined by 2, 4, 6-trinitrobenzene sulphonic acid assay. The effects of various PEGylation degrees on cellular toxicity and transfection ability of PEI polymer were assessed on BT-474 and MCF-10A cell lines. Compared to unmodified PEI, PEG-grafted PEI copolymers demonstrated reduced cytotoxicity, particularly at higher PEG grafting ratios. Among different PEG-grafted PEIs, the PEG–PEI copolymer which grafted to PEG at a molar ratio of 10:1 had the highest transfection efficiency in both cell lines. The findings of this Letter showed that these PEG-grafted PEI copolymers have desirable gene transfection efficiency and favourable biocompatibility for gene delivery.
Starting Page	1090
Ending Page	1095
Page Count	6
Volume Number	13
e-ISSN	17500443
Issue Number	Issue 8, Aug (2018)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/mnl/13/8
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/mnl.2017.0457
Journal	Micro & Nano Letters
Publisher Date	2018-08-01
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	2,4,6-trinitrobenzene Sulphonic Acid Assay Biochemistry Biomedical Material Cancer Cationic Charge Cationic Polymer Cellular Biophysics Cellular Toxicity Condensation Cytotoxicity DNA DNA-condensing Ability Elastomers Electrochemistry And Electrophoresis Gene Therapy Gene Therapy Application Gene Transfection Efficiency Genetics Molecular Biophysics Molecular Weight Nonviral Gene Delivery Agent Patient Care And Treatment PEG-grafted PEI Copolymers PEI Surface Charge Density PEI Surface Modification Physical Chemistry of Biomolecular Solutions And Condensed State Physics of Subcellular Structure Plastics Polyethylene Glycol Molecule Polyethylenimine Polymer Blends Preparation of Polymers And Plastics Structure of Polymers Surface Charging Toxicology
Content Type	Text
Resource Type	Article

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