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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Rong Zhang Jian Qin Hao Tian Weihua Si Taihong Wang Zewen Liu |
| Copyright Year | 2011 |
| Description | Author affiliation: Micro-Nano Technologies Research Center, Hunan University, China (Rong Zhang; Jian Qin; Taihong Wang) || School of Mechanical Engineering, Dalian University of Technology, China (Hao Tian) || Institute of Microelectronics, Tsinghua University, China (Weihua Si; Zewen Liu) |
| Abstract | Electrochemical behaviors of DNA(Deoxyribonucleic acid) are studied for exploring label free PCR(Polymerase Chain Reaction) detection used in miniaturized PCR system. PCR chips with three electrodes are fabricated with CMOS (Complementary Metal Oxide Semiconductor) compatible process. Micro electrodes with different metals successfully are obtained inside the micro PCR reaction cell for optimizing the electrochemical process. Both purified DNA solution diluted in PBS buffer and amplified DsRed DNA solution of n (n=0,4,8,12,20) cycles are used as the solution system. The experiment revealed that the redox peaks current difference between DsRed DNA solution of 4 cycles and 12 cycles is 50 nA. |
| Starting Page | 952 |
| Ending Page | 955 |
| File Size | 1891096 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781612847757 |
| e-ISBN | 9781612847771 |
| DOI | 10.1109/NEMS.2011.6017512 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2011-02-20 |
| Publisher Place | Taiwan |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | DNA Educational institutions Microelectrodes Biosensors Optical sensors Optical device fabrication PCR amplification electrochemical micro PCR DsRed DNA PCR product |
| Content Type | Text |
| Resource Type | Article |
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