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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Peigang Deng Chang, D.C. Yi-Kuen Lee Junwei Zhou Gang Li |
| Copyright Year | 2011 |
| Description | Author affiliation: KAUST-HKUST Joint Micro/Nanofluidics Lab, Clear Water Bay, Kowloon, Hong Kong (Peigang Deng; Yi-Kuen Lee) || Stem Cell and Regeneration Program, School of Biomedical Sciences, CUHK, Shatin, NT, Hong Kong (Junwei Zhou; Gang Li) || Department of Biology, HKUST, Clear Water Bay, Kowloon, Hong Kong (Chang, D.C.) |
| Abstract | Experimental study of electroporation of bone marrow mesenchymal stem cells (MSCs) at the single-cell level was carried out on a micro EP chip by using single electric rectangular pulse. The threshold values of the electrode potential and pulse width for gas bubble generation on the micro electrodes due to electrolysis of water were revealed as 4.5 volt and 100 µs, respectively. Quantitative EP study was performed with various electric field strengths for various pulse widths, ranging from 20µs to 15ms. Over 1,000 single-cell EP results were used to construct an EP “phase diagram”, which delineates the boundaries for (1) effective EP of MSCs and (2) electric cell lysis of MSCs. Finally, the micro EP chip showed successful transfection of the pEGFP-C1 plasmid into the MSCs by properly choosing the electric parameters from the EP “phase diagram”. |
| Starting Page | 96 |
| Ending Page | 99 |
| File Size | 1271885 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781612847757 |
| e-ISBN | 9781612847771 |
| DOI | 10.1109/NEMS.2011.6017304 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2011-02-20 |
| Publisher Place | Taiwan |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Electric fields Fluorescence Electrodes Electrochemical processes Electric potential Bones Media micro electroporation chip electroporation bone marrow mesenchymal stem cell EP phase diagram |
| Content Type | Text |
| Resource Type | Article |
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