Loading...
Please wait, while we are loading the content...
Similar Documents
Sequence-Independent DNA Adsorption on Few-Layered Oxygen-Functionalized Graphene Electrodes: An Electrochemical Study for Biosensing Application
| Content Provider | MDPI |
|---|---|
| Author | Asefifeyzabadi, Narges Holland, Torrey E. Sivakumar, Poopalasingam Talapatra, Saikat Senanayake, Ishani M. Goodson, Boyd M. Shamsi, Mohtashim H. |
| Copyright Year | 2021 |
| Description | DNA is strongly adsorbed on oxidized graphene surfaces in the presence of divalent cations. Here, we studied the effect of DNA adsorption on electrochemical charge transfer at few-layered, oxygen-functionalized graphene $(GO_{x}$) electrodes. DNA adsorption on the inkjet-printed $GO_{x}$ electrodes caused amplified current response from ferro/ferricyanide redox probe at concentration range 1 aM–10 nM in differential pulse voltammetry. We studied a number of variables that may affect the current response of the interface: sequence type, conformation, concentration, length, and ionic strength. Later, we showed a proof-of-concept DNA biosensing application, which is free from chemical immobilization of the probe and sensitive at attomolar concentration regime. We propose that $GO_{x}$ electrodes promise a low-cost solution to fabricate a highly sensitive platform for label-free and chemisorption-free DNA biosensing. |
| Starting Page | 273 |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios11080273 |
| Journal | Biosensors |
| Issue Number | 8 |
| Volume Number | 11 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2021-08-14 |
| Access Restriction | Open |
| Subject Keyword | Biosensors Dna Biosensors Graphene Electrodes Inkjet-printing Trinucleotide Repeats Label-free Electrochemical Biosensors |
| Content Type | Text |
| Resource Type | Article |
