NDLI: Design and fabrication of micro/bio chip for in vitro fabrication of toroidal cellular aggregates

Content Provider	IEEE Xplore Digital Library
Author	Chumtong, P. Kojima, M. Horade, M. Ohara, K. Kamiyama, K. Mae, Y. Akiyama, Y. Yamato, M. Arai, T.
Copyright Year	2014
Description	Author affiliation: Grad. Sch. of Eng. Sci., Osaka Univ., Toyonaka, Japan (Chumtong, P.; Kojima, M.; Horade, M.; Kamiyama, K.; Mae, Y.; Arai, T.) \|\| Fac. of Sci. & Technol., Meijo Univ., Nagoya, Japan (Ohara, K.) \|\| Inst. of Adv. Biomed. Eng. & Sci. at TWIns, Tokyo Women's Med. Univ., Tokyo, Japan (Akiyama, Y.; Yamato, M.)
Abstract	The unique geometry of toroidal cellular aggregate has caught much attention during last few years due to its variety of applications in tissue engineering. For example, the micro lumen of connected toroidal aggregates enables a communication channel for vascularization within complex cellular constructs. This channel significantly helps preventing the cell death, due to the lack of nutrients. Furthermore, the direct assembly of multiple toroidal aggregates results in the formation of tissue engineered blood vessel, which is highly needed for transplantation in clinical use. In this paper, we design and fabricate a micro/bio chip enabling the fabrication of toroidal cellular aggregates. The chip has a 3×3 array of deformable scaffolds. Each scaffold has a diameter of 500 μm, a depth of 250 μm, a 130 μm-diameter pillar located in the middle, and a 20 μm-thick membrane coated on the top surface. The temporary toroidal scaffold is generated by decompression before cell seeding, and maintained until the cellular structure is strong. The $1.5×10^{5}$ cells of NIH3T3 were seeded in a chip, and cellular formation of toroidal shape was observed after 3 days. The retrieved aggregates have an outer diameter of about 330 μm, a lumen diameter of about 40 μm, and a thickness of about 100 μm. The cell viability test confirms the healthy condition of the aggregates. Moreover, the noninvasive recovery of the toroidal aggregates provided by the elastic membrane of the scaffolds suggests effectiveness of using this chip in biological applications.
Starting Page	37
Ending Page	42
File Size	277378
Page Count	6
File Format	PDF
ISBN	9781479939787
e-ISBN	9781479939794
DOI	10.1109/ICMA.2014.6885668
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-08-03
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Aggregates Biomembranes Fabrication Microchannels Silicon Geometry Shape Tissue Engineering Biomedical device Microfabrication
Content Type	Text
Resource Type	Article

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