NDLI: High throughput mechanical characterization of oocyte using robot integrated microfluidic chip

Content Provider	IEEE Xplore Digital Library
Author	Sakuma, S. Turan, B. Arai, F.
Copyright Year	2013
Description	Author affiliation: Dept. of Mech. Sci. & Eng., Nagoya Univ., Nagoya, Japan (Arai, F.) \|\| Dept. of Micro - Nano Syst. Eng., Nagoya Univ., Nagoya, Japan (Sakuma, S.; Turan, B.)
Abstract	This paper presents a novel measurement system of cellular mechanical properties based on a robot integrated microfluidic chip. In order to achieve the high throughput measurement of cellular mechacal properties, we proposed the robot integrated microfluidic chip (robochip), taking advantages of both of micromechanical manipulator and Lab-on-a-Chip devices. The robochip contained a pair of a magnetically driven on-chip robotic probe and a force sensor. The characterization system based on the robochip performed by the visual feedback control, and the continuous cell measurement was demonstrated. The throughput of our system was 15 to 20 seconds per one oocyte. Moreover, the measurement of the viscoelastic properties were demonstrated as a quality evaluation of oocyte. Experimental results shows that the oocyte has the viscoelastic properties among the same culture condition, and it is important to analyze the mechanical properties of oocyte for the evaluation of the quality. From these results, we concluded that the high through put cellular mechanical characterization was achieved, and our robochip approach was a promising technique for a cellular characterization because the chip part was disposable.
Sponsorship	IEEE Ind. Electron. Soc.
Starting Page	2047
Ending Page	2052
File Size	1691377
Page Count	6
File Format	PDF
ISSN	21530858
DOI	10.1109/IROS.2013.6696630
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-11-03
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Probes System-on-chip Force Robot sensing systems Biomedical measurement Mechanical variables measurement
Content Type	Text
Resource Type	Article
Subject	Control and Systems Engineering Computer Science Applications Computer Vision and Pattern Recognition Software

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