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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Chessel, A. Pecot, T. Bardin, S. Kervrann, C. Salamero, J. |
| Copyright Year | 2009 |
| Description | Author affiliation: INRIA Centre Rennes - Bretagne Atlantique, F-35042 France (Chessel, A.; Pecot, T.; Kervrann, C.) || ¿Cell and Tissue Imaging Facility¿ - IBISA, Institut Curie, F-75248 Paris, France (Salamero, J.) || UMR 144 CNRS - Institut Curie, F-75248 Paris, France (Bardin, S.) |
| Abstract | A wide range of proteins viewed using xFP probes in fluorescence video-microscopy shows a cytosolic state diffusing slowly and a membrane state eventually moving as vesicles or small tubules along the cell cytoskeleton network. Due to the property of fluorescence, the measured intensity is the sum of the contributions of these two components. In this study we perform an evaluation of different methods for the automatic decomposition of the two additive components/states. Four algorithms are studied: i) gray-scale opening (a.k.a. “rolling ball”); ii) wavelet-based detection combined with image interpolation; iii) Conditional Random Field-based detection; iv) computational geometry for temporal signal analysis. The evaluation protocol, related to the actual use of image sequence decomposition, includes a qualitative evaluation on real image sequences by experts and a quantitative evaluation on simulated sequences that mimic real images. |
| Starting Page | 1099 |
| Ending Page | 1102 |
| File Size | 461076 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781424439317 |
| ISSN | 19457928 |
| DOI | 10.1109/ISBI.2009.5193248 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2009-06-28 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Image sequences Biomembranes Image analysis Image sequence analysis Algorithm design and analysis Fluorescence Additives Proteins Probes Performance evaluation algorithm review Fluorescence microscopy video additive decomposition |
| Content Type | Text |
| Resource Type | Article |
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