NDLI: RREB-1 is a transcriptional repressor of HLA-G.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Flajollet, Sébastien Carosella, Edgardo D. Moreau, Philippe Poras, Isabelle
Description	Country affiliation: France Author Affiliation: Flajollet S ( Commissariat à l'Energie Atomique, Direction des Sciences du Vivant, IBM, Service de Recherches en Hémato-Immunologie, Institut Universitaire d'Hématologie, Hôpital Saint-Louis, 75010 Paris, France.)
Abstract	The nonclassical HLA-G is a molecule specifically involved in immune tolerance with highly restricted tissue distribution in healthy conditions. Yet it is overexpressed in numerous tumors and in allografts with better acceptance. Major mechanisms involved in regulation of HLA-G transcription are still poorly described. Thus, to characterize these mechanisms we have developed a specific proteomic approach to identify proteins that bind differentially to the HLA-G gene promoter by promoter pull-down assay followed by spectrometry mass analysis. Among specific binding factors, we focused on RREB-1, a ras-responsive element binding protein 1. We demonstrated that RREB-1 represses HLA-G transcriptional activity and binds three ras response elements within the HLA-G promoter. RREB-1 protein, specifically in HLA-G-negative cells, interacts with subunits of CtBP complex implicated in chromatin remodeling. This demonstration is the first of a repressor factor of HLA-G transcriptional activity taking part in HLA-G repression by epigenetic mechanisms.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	11
Volume Number	183
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2009-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Dna-binding Proteins Genetics Gene Expression Regulation Hla Antigens Histocompatibility Antigens Class I Transcription Factors Blotting, Western Cell Line, Tumor Chromatin Immunoprecipitation Immunology Metabolism Electrophoresis, Gel, Two-dimensional Electrophoretic Mobility Shift Assay Epigenesis, Genetic Gene Expression Biosynthesis Hla-g Antigens Molecular Sequence Data Promoter Regions, Genetic Proteomics Rna Interference Reverse Transcriptase Polymerase Chain Reaction Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Transcription, Genetic Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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