NDLI: The impact of inoculation parameters on the pathogenesis of contact lens-related infectious keratitis.

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The impact of inoculation parameters on the pathogenesis of contact lens-related infectious keratitis.

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The impact of inoculation parameters on the pathogenesis of contact lens-related infectious keratitis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mun, James J. Evans, David J. Fleiszig, Suzanne M. J. Tam, Connie
Description	Country affiliation: United States Author Affiliation: Tam C ( School of Optometry, University of California, Berkeley, California 94720-2020, USA.)
Abstract	PURPOSE: Contact lens wear predisposes to Pseudomonas aeruginosa keratitis, but the mechanisms involved remain unclear. An in vivo model was used to study lens inoculation conditions enabling disease. METHODS: Custom-made hydrogel contact lenses were fitted to rats after incubation in P. aeruginosa approximately 10(11) cfu/mL (3 hours) or approximately 10(3) cfu/mL (24 hours). Another group was inadvertently inoculated with a suction pen previously used with high inocula, but rinsed in ethanol and stored dry (6 months). Some corneas were tissue paper-blotted to cause fluorescein staining before lens fitting. Contralateral eyes were untreated. Twenty-four hours after disease detection, lenses were transferred to naive rats or examined by confocal microscopy before homogenization to quantify viable bacteria. After lens removal, corneas were washed to collect nonadherent bacteria and were analyzed by immunohistochemistry. RESULTS: All eyes challenged with unworn contaminated lenses developed keratitis after approximately 7 to 10 days. Disease delay and severity were unaffected by inoculum parameters or tissue blotting but occurred sooner with lenses transferred from infected eyes ( approximately 2 days). Worn lenses and corneal washes contained infecting bacteria. Posterior, not anterior, lens surfaces harbored P. aeruginosa biofilms that penetrated the lens matrix. Diseased corneas showed an infiltration of phagocytes and T-lymphocytes. CONCLUSIONS: P. aeruginosa induces keratitis in this lens-wearing model after a single inoculation. Delayed disease onset was interesting considering the greater keratitis risk during extended wear. Infection did not require the disruption of corneal barrier function before lens wear and occurred without exposure to lens care solutions. The data suggest that keratitis involves biofilm formation or other bacterial adaptations in vivo.
ISSN	01460404
e-ISSN	15525783
DOI	10.1167/iovs.09-4593
Journal	Investigative Opthalmology & Visual Science
Issue Number	6
Volume Number	51
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2010-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Biofilms Contact Lenses, Hydrophilic Microbiology Corneal Ulcer Metabolism Eye Infections, Bacterial Pseudomonas Infections Pseudomonas Aeruginosa Physiology Animals Bacterial Adhesion Bacteriological Techniques Disease Models, Animal Fluorescent Antibody Technique, Indirect Hydrogel Microscopy, Confocal Rats, Inbred Lew Research Support, N.i.h., Extramural Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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