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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Orellana, Guillermo Haigh-Flórez, David de la Hera, Cristina Costas, Eduardo |
| Description | Country affiliation: Spain Author Affiliation: Haigh-Flórez D ( Chemical Optosensors and Applied Photochemistry Group (GSOLFA), Dpmt. of Organic Chemistry, Faculty of Chemistry, Complutense University of Madrid, E-28040 Madrid, Spain.); de la Hera C ( Microalgae Biotechnology: Production and Toxicity Group (ALBIOTOX), Dpmt. of Animal Production (Genetics), Faculty of Veterinary Medicine, Complutense University of Madrid, E-28040 Madrid, Spain.); Costas E ( Microalgae Biotechnology: Production and Toxicity Group (ALBIOTOX), Dpmt. of Animal Production (Genetics), Faculty of Veterinary Medicine, Complutense University of Madrid, E-28040 Madrid, Spain.); Orellana G ( Chemical Optosensors and Applied Photochemistry Group (GSOLFA), Dpmt. of Organic Chemistry, Faculty of Chemistry, Complutense University of Madrid, E-28040 Madrid, Spain. Electronic address: orellana@quim.ucm.es.) |
| Abstract | The microalgal species Dictyosphaerium chlorelloides (D. c.) was immobilized into porous silicone films and their photosynthetic activity was monitored with an integrated robust luminescent O2 sensor. The biosensor specificity towards a particular pesticide has been achieved by manufacturing a fiber-optic dual-head device containing both analyte-sensitive and analyte-resistant D. c. strains. The latter are not genetically modified microalgae, but a product of modified Luria-Delbrück fluctuation analysis followed by ratchet selection cycles. In this way the target herbicide decreases the O2 production of the analyte-sensitive immobilized strain without affecting the analyte-resistant population response; any other pollutant will lower the O2 production of both strains. The effect of the sample flow-rate, exposure time to the herbicide, biomass loading, biosensor film thickness, intensity of the actinic light, illumination cycle, and temperature on the biosensor response has been evaluated using waterborne simazine as test bench. The biosensing device is able to provide in situ measurements of the herbicide concentration every 180 min. The biosensor limit of detection for this herbicide was 12 µg L(-1), with a working range of 50-800 µg L(-1). The biosensor specificity to simazine has been assessed by comparing its response to that of isoproturon. |
| ISSN | 09565663 |
| Volume Number | 54 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Fiber Optic Technology Herbicides Analysis Microalgae Metabolism Oxygen Simazine Cells, Immobilized Cytology Equipment Design Limit Of Detection Luminescence Water Pollutants, Chemical Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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