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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Dardenne, Freddy Ahmad Rather, Jahangir Robbens, Johan Pilehvar, Sanaz De Wael, Karolien Blust, Ronny |
| Description | Country affiliation: Belgium Author Affiliation: Pilehvar S ( University of Antwerp, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium.); Ahmad Rather J ( University of Antwerp, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium.); Dardenne F ( University of Antwerp, Department of Biology, Groenenborgerlaan 171, 2020 Antwerp, Belgium.); Robbens J ( Institute for Agricultural and Fisheries Research, Ankerstraat 1, 8400 Ostend, Belgium.); Blust R ( University of Antwerp, Department of Biology, Groenenborgerlaan 171, 2020 Antwerp, Belgium.); De Wael K ( University of Antwerp, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium. Electronic address: Karolien.DeWael@uantwerpen.be.) |
| Abstract | A novel strategy to sense target molecules in human blood serum is achieved by immobilizing aptamers (APTs) on multi-walled carbon nanotubes (MWCNT) modified electrodes. In this work, the aminated aptamer selected for hydroxylated polychlorinated biphenyl (OH-PCB) was covalently immobilized on the surface of the MWCNT-COOH modified glassy carbon electrode through amide linkage. The aptamers function as recognition probes for OH-PCB by the binding induced folding of the aptamer. The developed aptasensing device was characterized by electrochemical impedance spectroscopy (EIS), atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). The aptasensor displayed excellent performance for OH-PCB detection with a linear range from 0.16 to 7.5 µM. The sensitivity of the developed aptasensing platform is improved (1×10(-8) M) compared to the published report (1×10(-6) M) for the determination of OH-PCB (Turner et al., 2007). The better performance of the sensor is due to the unique platform, i.e. the presence of APTs onto electrodes and the combination with nanomaterials. The aptamer density on the electrode surface was estimated by chronocoulometry and was found to be 1.4×10(13) molecules cm(-2). The validity of the method and applicability of the aptasensor was successfully evaluated by the detection of OH-PCB in a blood serum sample. The described approach for aptasensing opens up new perspectives in the field of biomonitoring providing a device with acceptable stability, high sensitivity, good accuracy and precision. |
| ISSN | 09565663 |
| Volume Number | 54 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-04-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aptamers, Nucleotide Chemistry Biosensing Techniques Instrumentation Nanotubes, Carbon Polychlorinated Biphenyls Blood Dielectric Spectroscopy Electrochemical Techniques Electrodes Humans Hydroxylation Limit Of Detection Microscopy, Atomic Force Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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