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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Peng, Lu Zhu, Zhi Chen, Yan Han, Da Tan, Weihong |
| Description | Country affiliation: United States Author Affiliation: Peng L ( Center for Research at Bio/nano Interface, Department of Chemistry, University of Florida, Gainesville, FL 32611-7200, USA.) |
| Abstract | We have developed a novel DNA assay based on exonuclease III (ExoIII)-induced target recycling and the fluorescence quenching ability of graphene oxide (GO). This assay consists of a linear DNA probe labeled with a fluorophore in the middle. Introduction of target sequence induces the exonuclease III catalyzed probe digestion and generation of single nucleotides. After each cycle of digestion, the target is recycled to realize the amplification. Finally, graphene oxide is added to quench the remaining probes and the signal from the resulting fluorophore labeled single nucleotides is detected. With this approach, a sub-picomolar detection limit can be achieved within 40 min at 37°C. The method was successfully applied to multicolor DNA detection and the analysis of telomerase activity in extracts from cancer cells. |
| ISSN | 09565663 |
| e-ISSN | 18734235 |
| DOI | 10.1016/j.bios.2012.03.002 |
| Journal | Biosensors and Bioelectronics |
| Issue Number | 1 |
| Volume Number | 35 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Biosensing Techniques Exodeoxyribonucleases Statistics & Numerical Data Genetics Dna Probes Graphite Hela Cells Limit Of Detection Nucleic Acid Amplification Techniques Sensitivity And Specificity Telomerase Evaluation Studies Research Support, N.i.h., Extramural Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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