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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chinnadayyala, Somasekhar R. Kakoti, Ankana Goswami, Pranab Santhosh, Mallesh |
| Description | Country affiliation: India Author Affiliation: Santhosh M ( Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.); Chinnadayyala SR ( Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.); Kakoti A ( Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.); Goswami P ( Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: pgoswami@iitg.ernet.in.) |
| Abstract | We report here a fluorescence quenching based non-enzymatic method for sensitive and reliable detection of free bilirubin in blood serum samples using human serum albumin (HSA) stabilized gold nanoclusters (HSA-AuNCs) as fluorescent probe. The fluorescence of the nanoclusters was strongly quenched by bilirubin in a concentration dependent manner by virtue of the inherent specific interaction between bilirubin and HSA. A strong binding constant of 0.55×10(6) L mole(-1) between the HSA-AuNC and bilirubin was discerned. The nano clusters each with size ~1.0 nm (in diameter) and a core of Au18 were homogeneously distributed in HSA molecules as revealed from the respective high resolution transmission electron microscopic and mass spectroscopic studies. The fluorescence quenching phenomena which obeyed a simple static quenching mechanism, was utilized for interference free detection of bilirubin with minimum detection limit (DL) of 248±12 nM (S/N=3). The fluorescence response of HSA-AuNCs against bilirubin was practically unaltered over a wide pH (6-9) and temperature (25-50 °C) range. Additionally, peroxidase-like catalytic activity of these nanoclusters was exploited for colorimetric detection of bilirubin in serum sample with a DL of 200±19 nM by following the decrease in absorbance (at λ440 nm) of the reaction and its rate constant (Kp) of 2.57±0.63 mL µg(-1) min(-1). Both these fluorometric and colorimetric methods have been successfully used for detection of free bilirubin in blood serum samples. |
| ISSN | 09565663 |
| Volume Number | 59 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bilirubin Blood Biosensing Techniques Methods Gold Chemistry Metal Nanoparticles Serum Albumin Colorimetry Humans Limit Of Detection Ultrastructure Models, Molecular Peroxidase Spectrometry, Fluorescence Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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