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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Chang Sup Shin, Hwa Hui Seo, Jeong Hyun Hwang, Byeong Hee Cha, Hyung Joon |
| Description | Author Affiliation: Shin HH ( Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea.); Seo JH ( School of Chemical Engineering, Yeungnam University, Gyeongsan 712-749, Republic of Korea.); Kim CS ( School of Chemistry and Biochemistry, Yeungnam University, Gyeongsan 712-749 Republic of Korea.); Hwang BH ( Division of Bioengineering, Incheon National University, Incheon 406-772, Republic of Korea.); Cha HJ ( Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea. Electronic address: hjcha@postech.ac.kr.) |
| Abstract | Life-threatening diarrheal cholera is usually caused by water or food contaminated with cholera toxin-producing Vibrio cholerae. For the prevention and surveillance of cholera, it is crucial to rapidly and precisely detect and identify the etiological causes, such as V. cholerae and/or its toxin. In the present work, we propose the use of a hybrid double biomolecular marker (DBM) microarray containing 16S rRNA-based DNA capture probe to genotypically identify V. cholerae and GM1 pentasaccharide capture probe to phenotypically detect cholera toxin. We employed a simple sample preparation method to directly obtain genomic DNA and secreted cholera toxin as target materials from bacterial cells. By utilizing the constructed DBM microarray and prepared samples, V. cholerae and cholera toxin were detected successfully, selectively, and simultaneously; the DBM microarray was able to analyze the pathogenicity of the identified V. cholerae regardless of whether the bacteria produces toxin. Therefore, our proposed DBM microarray is a new effective platform for identifying bacteria and analyzing bacterial pathogenicity simultaneously. |
| ISSN | 09565663 |
| Volume Number | 79 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Cholera Toxin Isolation & Purification Cholera Diagnosis Vibrio Cholerae Carbohydrates Chemistry Genetics Microbiology Dna Probes Genotype Humans Microarray Analysis Phenotype Rna, Ribosomal, 16s Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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