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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Del Valle, María A. Hernández, Loreto A. Armijo, Francisco |
| Description | Country affiliation: Chile Author Affiliation: Hernández LA ( Pontificia Universidad Católica de Chile, Facultad de Química, Departamento de Química Inorgánica, Laboratorio de Electroquímica de Polímeros (LEP), Vicuña Mackenna 4860, 7820436 Macul, Santiago, Chile.); Del Valle MA ( Pontificia Universidad Católica de Chile, Facultad de Química, Departamento de Química Inorgánica, Laboratorio de Electroquímica de Polímeros (LEP), Vicuña Mackenna 4860, 7820436 Macul, Santiago, Chile. Electronic address: mdvalle@uc.cl.); Armijo F ( Pontificia Universidad Católica de Chile, Facultad de Química, Departamento de Química Inorgánica, Laboratorio de Electroquímica de Polímeros (LEP), Vicuña Mackenna 4860, 7820436 Macul, Santiago, Chile.) |
| Abstract | The detection of naturally occurring desoxyribonucleic acid (DNA) has become a subject of study by the projections that would generate to be able to sense the genetic material for the detection of future diseases. Bearing this in mind, to provide new measuring strategies, in the current work the preparation of a low-cost electrode, modified with poly(1-amino-9,10-anthraquinone) nanowires using a SiO2 template, is carried out; the assembly is next modified by covalently attaching ssDNA strands. It must be noted that all this is accomplished by using solely electrochemical techniques, according to methodology developed for this purpose. SEM images of the modified surface show high order and homogeneity in the distribution of modified nanowires over the electrode surface. In turn, after the hybridization with its complementary strand, the voltammetric responses enable corroborating the linear relationship between hybridization at different DNA concentrations and normalized current response, obtaining a limit of detection (LOD) 5.7·10(-12)gL(-1) and limit of quantification (LOQ) 1.9·10(-11)gL(-1). The working dynamic range is between 1.4·10(-7) and 8.5·10(-9)gL(-1) with a correlation coefficient 0.9998. The successful obtaining of the modified electrode allows concluding that the high order reached by the nanostructures, guides the subsequent single strand of DNA (ssDNA) covalent attachment, which after hybridization with its complementary strand brings about a considerable current increase. This result allows foreseeing a guaranteed breakthrough with regard to the use of the biosensor in real samples. |
| ISSN | 09565663 |
| Volume Number | 79 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Dna Isolation & Purification Electrochemical Techniques Methods Quinones Chemistry Anthraquinones Limit Of Detection Nanostructures Nucleic Acid Hybridization Silicon Dioxide Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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