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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ford, Nicole Zhen, Le Rorrer, Gregory L. Roesijadi, Guritno Gale, Debra K. |
| Description | Author Affiliation: Zhen L ( School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, Oregon, 97331 USA.); Ford N ( Marine Sciences Laboratory, Pacific Northwest National Laboratory, Sequim, WA, 98382 USA.); Gale DK ( School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, Oregon, 97331 USA.); Roesijadi G ( Marine Sciences Laboratory, Pacific Northwest National Laboratory, Sequim, WA, 98382 USA); Rorrer GL ( School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, Oregon, 97331 USA. Electronic address: rorrergl@engr.orst.edu.) |
| Abstract | A selective and label-free biosensor for detection of the explosive compound 2,4,6-trinitrotoluene (TNT) in aqueous solution was developed based on the principle of photoluminescence quenching of upon immunocomplex formation with antibody-functionalized diatom frustule biosilica. The diatom frustule is an intricately nanostructured, highly porous biogenic silica material derived from the shells of microscopic algae called diatoms. This material emits strong visible blue photoluminescence (PL) upon UV excitation. PL-active frustule biosilica was isolated from cultured cells of the marine diatom Pinnularia sp. and functionalized with a single chain variable fragment (scFv) derived from an anti-TNT monoclonal antibody. When TNT was bound to the anti-TNT scFv-functionalized diatom frustule biosilica, the PL emission from the biosilica was partially quenched due to the electrophilic nature of the nitro (-NO2) groups on the TNT molecule. The dose-response curve for immunocomplex formation of TNT on the scFv-functionalized diatom frustule biosilica had a half-saturation binding constant of 6.4 ± 2.4·10(-8)M and statistically-significant measured detection limit of 3.5·10(-8)M. The binding and detection were selective for TNT and TNB (trinitrobenzene) but not RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine) or 2,6-DNT (2,6-dinitrotoluene). |
| ISSN | 09565663 |
| Volume Number | 79 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antibodies, Monoclonal Chemistry Diatoms Explosive Agents Analysis Nanostructures Trinitrotoluene Water Pollutants, Chemical Water Antibodies, Immobilized Biosensing Techniques Methods Ultrastructure Dinitrobenzenes Limit Of Detection Luminescent Measurements Silicon Dioxide Single-chain Antibodies Evaluation Studies Journal Article Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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