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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Beloglazova, Natalia V. Aubert, Tangi De Saeger, Sarah Hens, Zeger Van Deun, Rik Goftman, Valentina V. Ginste, Dries Vande Goryacheva, Irina Yu |
| Description | Author Affiliation: Goftman VV ( Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia); Aubert T ( Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.); Ginste DV ( IBCN/Electromagnetics Group, Department of Information Technology, Ghent University/iMinds, Sint-Pietersnieuwstraat 41, B-9000 Gent, Belgium.); Van Deun R ( Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.); Beloglazova NV ( Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.); Hens Z ( Department of Inorganic and Physical Chemistry, Ghent University, Krijgslaan 281 S3, 9000 Ghent, Belgium.); De Saeger S ( Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium.); Goryacheva IY ( Department of General and Inorganic Chemistry, Chemistry Institute, Saratov State University, Astrakhanskaya 83, 410012 Saratov, Russia) |
| Abstract | To create bright and stable fluorescent biolabels for immunoassay detection of mycotoxin deoxynivalenol in food and feed, CdSe/CdS/ZnS core-shell quantum dots (QDs) were encapsulated in silica nanoparticles through a water-in-oil reverse microemulsion process. The optical properties and stability of the obtained silica coated QDs (QD@SiO2), modified with amino, carboxyl and epoxy groups and stabilized with polyethylene glycol fragments, were characterized in order to assess their bioapplicability. The developed co-condensation techniques allowed maintaining 80% of the initial fluorescent properties and yielded stable fluorescent labels that could be easily activated and bioconjugated. Further, the modified QD@SiO2 were efficiently conjugated with antibodies and applied as a novel label in a microtiter plate based immunoassay and a quantitative column-based rapid immunotest for deoxynivalenol detection with IC50 of 473 and 20 ng/ml, respectively. |
| ISSN | 09565663 |
| Volume Number | 79 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antibodies, Immobilized Chemistry Fluorescent Dyes Food Analysis Methods Immunoassay Quantum Dots Silicon Dioxide Trichothecenes Analysis Animal Feed Microbiology Animals Food Microbiology Humans Polyethylene Glycols Ultrastructure Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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