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Phenotypic and genetic characterization of Vibrio cholerae O1 clinical isolates collected through national antimicrobial resistance surveillance network in Nepal

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Phenotypic and genetic characterization of Vibrio cholerae O1 clinical isolates collected through national antimicrobial resistance surveillance network in Nepal

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shakya, Geeta Kim, Dong Wook Clemens, John D. Malla, Sarala Upadhyaya, Bishnu Prasad Dumre, Shyam Prakash Shrestha, Sirjana Devi Adhikari, Shailaja Sharma, Supriya Rijal, Nisha Shrestha, Sanjaya K. Mason, Carl Kansakar, Palpasa
Spatial Coverage	Nepal
Description	Country affiliation: Nepal Author Affiliation: Shakya G ( National Public Health Laboratory, Kathmandu, Nepal.)
Abstract	Cholera occurs in sporadic cases and outbreaks in Nepal each year. Vibrio cholerae O1 (n = 522) isolated during 2007–2010 from diarrheal patients at 10 different hospital laboratories in Nepal were characterized. Biochemical and serologic identifications showed that all the isolates belonged to serogroup O1, El Tor biotype. Except 72 isolates of Inaba serotype isolated in the year 2007, all the remaining isolates were of Ogawa serotype. All isolates were resistant to nalidixic acid and furazolidone. Resistance to tetracycline, ciprofloxacin, erythromycin and co-trimoxazole were 21, 4, 16 and 90 % respectively. Seventy-seven of these isolates were selected for further characterization for ctxB gene and MLVA typing. Two different variants of classical type cholera toxin were observed. Ogawa strains from 2007 and 2010-Western Nepal outbreak harbored CTX-3 type cholera toxin, whereas Inaba serotypes in 2007 and the remaining Ogawa serotypes in 2008–2010 harbored CTX 3b-type toxin. MLVA analysis showed circulation of four different groups of altered V. cholerae O1 El Tor strains. Two different profiles were seen among 2007 Inaba (9, 3, 6, x, x) and Ogawa (10, 7, 6, x, x) isolates. The MLVA profile of 2008 and 2009 Ogawa isolates were similar to those of Inaba strains of 2007. Isolates from 2010 also showed three different MLVA profiles; profile 9, 3, 6, x, x in 3 isolates, 11, 7, 6, x, x among 2010 Western Nepal outbreak strains and profile 8, 3, 6, x, x among isolates from Butwal and Kathmandu.
File Format	HTM / HTML
ISSN	09593993
Issue Number	8
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-08-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Epidemics Research Support, Non-u.s. Gov't Discipline Microbiology Microbiology Humans Isolation & Purification Drug Therapy Drug Resistance, Bacterial Epidemiology Vibrio Cholerae O1 Drug Effects Journal Article Discipline Biotechnology Phenotype Molecular Epidemiology Dna, Bacterial Base Sequence Classification Cholera Toxin Genetics Cholera Nepal Serotyping
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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Phenotypic and genetic characterization of Vibrio cholerae O1 clinical isolates collected through national antimicrobial resistance surveillance network in Nepal