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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fang, Yuzhi Wang, Qingjiang He, Pingang Chen, Hong Fan, Huajun Jiao, Fang Zhang, Fan |
| Description | Author Affiliation: Fan H ( Department of Chemistry, East China Normal University, Shanghai 200241, PR China.) |
| Abstract | Qualitative and quantitative detection of DNA was achieved by a 'sandwich' DNA sensor through SG/TC (substrate generation and tip collection) mode of scanning electrochemical microscopy (SECM). The 'sandwich' DNA structure was formed by the hybridization of thiol-tethered oligodeoxynucleotide probes (capture probe), assembled on the gold substrate surface, with target DNA and biotinylated indicator probe. HRP (horseradish peroxidase)-wrapped SiO2 nanoparticles were linked to the sandwich structure through biotin-streptavidin interaction. Hydroquinone (H2Q) was oxidized to benzoquinone (BQ) at the modified substrate surface where sequence-specific hybridization had occurred through the HRP-catalyzed reaction in the presence of H2O2. The detection was based on the reduction of BQ generated on the modified substrate by SECM tip. For SECM imaging experiment, we structured the microsensor platform through localized desorption of 1-dodecanethiol monolayer. Approach curves were employed for quantitative detection of DNA concentration. The detection limit of complementary DNA was as low as 0.8pM. This technique is promising for the application on electrochemical DNA chip. |
| ISSN | 09565663 |
| Volume Number | 47 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-09-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Dna Isolation & Purification Silicon Dioxide Chemistry Electrochemical Techniques Horseradish Peroxidase Hydrogen Peroxide Microscopy, Electrochemical, Scanning Nanoparticles Nucleic Acid Hybridization Sulfhydryl Compounds Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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