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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lian, Wenjing Liu, Hongyun Yu, Xue Zhang, Jiannan |
| Description | Author Affiliation: Yu X ( College of Chemistry, Beijing Normal University, 19, Xinjiekouwai Street, Haidian District, Beijing 100875, People's Republic of China.); Lian W ( College of Chemistry, Beijing Normal University, 19, Xinjiekouwai Street, Haidian District, Beijing 100875, People's Republic of China.); Zhang J ( Beijing No. 55 High School, Beijing 100027, People's Republic of China.); Liu H ( College of Chemistry, Beijing Normal University, 19, Xinjiekouwai Street, Haidian District, Beijing 100875, People's Republic of China) |
| Abstract | Herein, poly(N-isopropylacrylamide-co-N,N'-dimethylaminoethylmethacrylate) copolymer films were polymerized on electrode surface with a simple one-step method, and the enzyme horseradish peroxidase (HRP) was embedded in the films simultaneously, which were designated as P(NiPAAm-co-DMEM)-HRP. The films exhibited a reversible structure change with the external stimuli, such as pH, CO2, temperature and SO4(2-), causing the cyclic voltammetric (CV) response of electroactive K3Fe(CN)6 at the film electrodes to display the corresponding multi-stimuli sensitive ON-OFF behavior. Based on the switchable CV property of the system and the electrochemical reduction of H2O2 catalyzed by HRP in the films and mediated by Fe(CN)6(3-) in solution, a 5-input/3-output logic gate was established. To further increase the complexity of the logic system, another enzyme glucose oxidase (GOD) was added into the films, designated as P(NiPAAm-co-DMEM)-HRP-GOD. In the presence of oxygen, the oxidation of glucose in the solution was catalyzed by GOD in the films, and the produced H2O2 in situ was recognized and electrocatalytically reduced by HRP and mediated by Fe(CN)6(3-). Based on the bienzyme films, a cascaded or concatenated 4-input/3-output logic gate system was proposed. The present work combined the multi-responsive interface with bioelectrocatalysis to construct cascaded logic circuits, which might open a new avenue to develop biocomputing elements with more sophisticated functions and design novel glucose biosensors. |
| ISSN | 09565663 |
| Volume Number | 80 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-06-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Enzymes, Immobilized Chemistry Glucose Isolation & Purification Electrodes Glucose Oxidase Horseradish Peroxidase Humans Hydrogen Peroxide Oxidation-reduction Oxygen Polymers Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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