NDLI: Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor.

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Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor.

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Hetero-enzyme-based two-round signal amplification strategy for trace detection of aflatoxin B1 using an electrochemical aptasensor.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Wei Zheng, Wanli Teng, Jun Ye, Yingwang Liu, Guodong Xue, Feng Wu, Jingjing Cheng, Lin Pan, Daodong
Description	Country affiliation: China Author Affiliation: Zheng W ( School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials and Devices, Hefei University of Technology, Hefei 23009, China.); Teng J ( School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials and Devices, Hefei University of Technology, Hefei 23009, China.); Cheng L ( School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials and Devices, Hefei University of Technology, Hefei 23009, China.); Ye Y ( School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials and Devices, Hefei University of Technology, Hefei 23009, China.); Pan D ( School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials and Devices, Hefei University of Technology, Hefei 23009, China.); Wu J ( School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials and Devices, Hefei University of Technology, Hefei 23009, China.); Xue F ( Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 200002, China. Electronic address: xuef1@jsciq.gov.cn.); Liu G ( Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58102, USA.); Chen W ( School of Biotechnology and Food Engineering, Anhui Provincial Key Lab of Functional Materials and Devices, Hefei University of Technology, Hefei 23009, China. Electronic address: chenweishnu@163.com.)
Abstract	An electrochemical aptasensor for trace detection of aflatoxin B1 (AFB1) was developed by using an aptamer as the recognition unit while adopting the telomerase and EXO III based two-round signal amplification strategy as the signal enhancement units. The telomerase amplification was used to elongate the ssDNA probes on the surface of gold nanoparticles, by which the signal response range of the signal-off model electrochemical aptasensor could be correspondingly enlarged. Then, the EXO III amplification was used to hydrolyze the 3'-end of the dsDNA after the recognition of target AFB1, which caused the release of bounded AFB1 into the sensing system, where it participated in the next recognition-sensing cycle. With this two-round signal amplified electrochemical aptasensor, target AFB1 was successfully measured at trace concentrations with excellent detection limit of 0.6*10(-4)ppt and satisfied specificity due to the excellent affinity of the aptamer against AFB1. Based on this designed two-round signal amplification strategy, both the sensing range and detection limit were greatly improved. This proposed ultrasensitive electrochemical aptasensor method was also validated by comparison with the classic instrumental methods. Importantly, this hetero-enzyme based two-round signal amplified electrochemical aptasensor offers a great promising protocol for ultrasensitive detection of AFB1 and other mycotoxins by replacing the core recognition sequence of the aptamer.
ISSN	09565663
Volume Number	80
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2016-06-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aflatoxin B1 Isolation & Purification Biosensing Techniques Electrochemical Techniques Metal Nanoparticles Chemistry Aptamers, Nucleotide Dna Exodeoxyribonucleases Gold Limit Of Detection Telomerase Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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