NDLI: Label-free impedimetric biosensor for Salmonella Typhimurium detection based on poly [pyrrole-co-3-carboxyl-pyrrole] copolymer supported aptamer.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sheikhzadeh, E. Jager, E. W. H. Chamsaz, M. Beni, V. Turner, A. P. F.
Description	Country affiliation: Iran Author Affiliation: Sheikhzadeh E ( Biosensors & Bioelectronics Centre, Dept. of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden); Chamsaz M ( Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.); Turner AP ( Biosensors & Bioelectronics Centre, Dept. of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.); Jager EW ( Biosensors & Bioelectronics Centre, Dept. of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.); Beni V ( Biosensors & Bioelectronics Centre, Dept. of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden. Electronic address: valerio.beni@acreo.se.)
Abstract	The Gram-negative bacterium, Salmonella Typhimurium (S. Typhimurium) is a food borne pathogen responsible for numerous hospitalisations and deaths all over the world. Conventional detection methods for pathogens are time consuming and labour-intensive. Hence, there is considerable interest in faster and simpler detection methods. Polypyrrole-based polymers, due to their intrinsic chemical and electrical properties, have been demonstrated to be valuable candidates for the fabrication of chemo/biosensors and functional surfaces. Similarly aptamers have been shown to be good alternatives to antibodies in the development of affinity biosensors. In this study, we report on the combination of poly [pyrrole-co-3-carboxyl-pyrrole] copolymer and aptamer for the development of a label-less electrochemical biosensor suitable for the detection of S. Typhimurium. Impedimetric measurements were facilitated by the effect of the aptamer/target interaction on the intrinsic conjugation of the poly [pyrrole-co-3-carboxyl-pyrrole] copolymer and subsequently on its electrical properties. The aptasensor detected S. Typhimurium in the concentration range 10(2)-10(8) CFU mL(-1) with high selectivity over other model pathogens and with a limit of quantification (LOQ) of 100 CFU mL(-1) and a limit of detection (LOD) of 3 CFU mL(-1). The suitability of the aptasensor for real sample detection was demonstrated via recovery studies performed in spiked apple juice samples. We envisage this to be a viable approach for the inexpensive and rapid detection of pathogens in food, and possibly in other environmental samples.
ISSN	09565663
Journal	Biosensors and Bioelectronics
Volume Number	80
e-ISSN	18734235
Language	English
Publisher	Elsevier
Publisher Date	2016-06-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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