NDLI: Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb(2+) using molecular beacon and DNAzyme based amplification strategy.

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Enzyme-free and label-free ultra-sensitive colorimetric detection of Pb(2+) using molecular beacon and DNAzyme based amplification strategy.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yun, Wen Jiang, JiaoLai Sang, Ge Cai, Dingzhou Zhao, Pengxiang Huang, Yu
Description	Author Affiliation: Yun W ( Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.); Cai D ( Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.); Jiang J ( Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.); Zhao P ( Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China.); Huang Y ( Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, No. 266 Fangzheng Avenue, Shuitu Hi-Tech Park, Shuitu Town, Beibei District, Chongqing 400714, PR China. Electronic address: huangyu@cigit.ac.cn.); Sang G ( Science and Technology on Surface Physics and Chemistry Laboratory, P.O. Box No. 9-35, Jiangyou City, Sichuan 621908, PR China. Electronic address: yunwenyunwen@qq.com.)
Abstract	An enzyme-free and label-free colorimetric Pb(2+) sensor based on DNAzyme and molecular beacon (MB) has been developed and demonstrated by recycle using enzyme strand for signal amplification. The substrate strand DNA (S-DNA) of DNAzyme could be converted into MB structure with base pairs of stem part at the both ends. The MB could hybridize with enzyme strand DNA (E-DNA) to form DNAzyme, and be activated and cleaved in the presence of Pb(2+). The cleaved MB is much less stable, releasing from the DNAzyme as two product pieces. The product pieces of MB are flexible and could bind to unmodified AuNPs to effectively stabilize them against salt-induced aggregation. Then, the E-DNA is liberated to catalyze the next reaction and amplify the response signal. By taking advantage of repeated using of E-DNA, our proposed method exhibited high sensitive for Pb(2+) detection in a linear range from 0.05 to 5 nM with detection limit of 20 pM by UV-vis spectrometer. Moreover, this method was also used for determination of Pb(2+) in river water samples with satisfying results. Importantly, this strategy could reach high sensitivity without any modification and complex enzymatic or hairpins based amplification procedures.
ISSN	09565663
Journal	Biosensors and Bioelectronics
Volume Number	80
e-ISSN	18734235
Language	English
Publisher	Elsevier
Publisher Date	2016-06-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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