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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Jiamian Zhao, Wei Yuan, Jingli Meng, Changgong Song, Jie Zhao, Yanqiu Wang, Xiuyun Wu, Shuo Gao, Zhanming |
| Description | Author Affiliation: Song J ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.); Wang J ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.); Wang X ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.); Zhao W ( School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.); Zhao Y ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.); Wu S ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China. Electronic address: wushuo@dlut.edu.cn.); Gao Z ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.); Yuan J ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.); Meng C ( School of Chemistry, Dalian University of Technology, Dalian 116023, PR China.) |
| Abstract | A highly sensitive signal-on photoelectrochemical (PEC) immunosensor was fabricated here using CdS:Mn/TiO2 as photoelectrochemical sensing platform, and silver nanoclusters and graphene naocomposites (AgNCs-GR) as signal amplification tags. The immunosensor was constructed based on the specific sandwich immunoreaction, and the photo-to-current conversion efficiency of the isolated protein modified CdS:Mn/TiO2 matrix was improved based on the synergistic effect of AgNCs-GR. Under irradiation, the photogenerated electrons from the AgNCs at a higher conduction band edge level could be transport to the CdS:Mn/TiO2 matrix with the assistance of highly conductive graphene nanosheets, as well as recycle the trapped excitons in the defects-rich CdS:Mn/TiO2 interface. The electron transport and exciton recycle reduced the possibility of electron-hole recombination and greatly improved the photo-to-current conversion efficiency of the sensing matrix. Based on the signal enhancement, a signal-on PEC immunosensors was fabricated for the detection of carcinoembryonic antigen (CEA), a model analyte related to many malignant diseases. Under optimal conditions, the as-prepared immunosensor showed excellent analytical performance, with a wide linear range from 1.0 pg/mL to 100 ng/mL and a low limit of detection of 1.0 pg/mL. The signal-on mode provided 2.48 times higher sensitivity compared with signal-off mode. This strategy demonstrated good accuracy and high selectivity for practical sample analysis, thus may have great application prospective in the prediction and early diagnosis of diseases. |
| ISSN | 09565663 |
| Volume Number | 80 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-06-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Carcinoembryonic Antigen Isolation & Purification Electrochemical Techniques Immunoassay Graphite Chemistry Humans Limit Of Detection Nanocomposites Quantum Dots Silver Titanium Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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