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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tiwari, Ashutosh Moloto, Harry Osikoya, Adeniyi Olugbenga Murugan, N. Arul Turner, Anthony Pf Dikio, Ezekiel Dixon Parlak, Onur Uzun, Lokman |
| Description | Country affiliation: South Africa Author Affiliation: Osikoya AO ( Biosensors and Bioelectronics Centre, IFM, Linköping University, 58183 Linköping, Sweden); Parlak O ( Biosensors and Bioelectronics Centre, IFM, Linköping University, 58183 Linköping, Sweden.); Murugan NA ( Virtual Laboratory for Molecular Probes, Division of Theoretical Chemistry and Biology, School of Biotechnology, Royal Institute of Technology, S-106 91 Stockholm, Sweden.); Dikio ED ( Applied Chemistry and Nanoscience Laboratory, Department of Chemistry, Vaal University of Technology, Private Bag X021, Vanderbijlpark, South Africa.); Moloto H ( Applied Chemistry and Nanoscience Laboratory, Department of Chemistry, Vaal University of Technology, Private Bag X021, Vanderbijlpark, South Africa.); Uzun L ( Biosensors and Bioelectronics Centre, IFM, Linköping University, 58183 Linköping, Sweden); Turner AP ( Biosensors and Bioelectronics Centre, IFM, Linköping University, 58183 Linköping, Sweden.); Tiwari A ( Biosensors and Bioelectronics Centre, IFM, Linköping University, 58183 Linköping, Sweden) |
| Abstract | In this study, we have demonstrated the use of chemical vapour deposition (CVD) grown-graphene to develop a highly-ordered graphene-enzyme electrode for electrochemical biosensing. The graphene sheets were deposited on 1.00mm thick copper sheet at 850°C using acetylene (C H ) as carbon source in an argon (Ar) and nitrogen (N ) atmosphere. An anionic surfactant was used to increase wettability and hydrophilicity of graphene; thereby facilitating the assembly of biomolecules on the electrode surface. Meanwhile, the theoretical calculations confirmed the successful modification of hydrophobic nature of graphene through the anionic surface assembly, which allowed high-ordered immobilisation of glucose oxidase (GOx) on the graphene. The electrochemical sensing activities of the graphene-electrode was explored as a model for bioelectrocatalysis. The bioelectrode exhibited a linear response to glucose concentration ranging from 0.2 to 9.8mM, with sensitivity of 0.087µA/µM/cm and a detection limit of 0.12µM (S/N=3). This work sets the stage for the use of acetylene-sourced CVD-grown graphene as a fundamental building block in the fabrication of electrochemical biosensors and other bioelectronic devices. |
| ISSN | 09565663 |
| Issue Number | Pt 1 |
| Volume Number | 89 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Aspergillus Niger Enzymology Biosensing Techniques Instrumentation Electrochemical Techniques Enzymes, Immobilized Chemistry Glucose Oxidase Graphite Acetylene Methods Electrodes Equipment Design Glucose Analysis Limit Of Detection Models, Molecular Surface-active Agents Volatilization Wettability Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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