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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Muthuraj, B. Mukherjee, Sudip Chowdhury, Sayan Roy Patra, Chitta Ranjan Iyer, Parameswar K. |
| Description | Country affiliation: India Author Affiliation: Muthuraj B ( Department of Chemistry, Indian Institute of Technology Guwahati, 781039 Assam, India.); Mukherjee S ( Biomaterials Group, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad 500007, Telangana, India); Chowdhury SR ( Department of Chemistry, Indian Institute of Technology Guwahati, 781039 Assam, India.); Patra CR ( Biomaterials Group, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad 500007, Telangana, India); Iyer PK ( Department of Chemistry, Indian Institute of Technology Guwahati, 781039 Assam, India) |
| Abstract | A strategy to develop water soluble, biocompatible nanocomposite probe for the detection of pyrophosphate (PPi) in physiological conditions and in in vitro live melanoma cancer cells (B16F10) is reported. The self-assembled nanocomposite probe comprised of amino acid (histidine) functionalized perylenediimide (PDI-HIS), copper ion and graphene oxide (GO) and that could be utilized as a highly effective sensing platform in biological conditions and cellular environment via fluorescence 'turn-on' for PPi detection. This controlled fabrication of metal organic self-assembled spheres along with GO proved very valuable for the detection of PPi in unprecedented sensitivity over other competing ions. The PDI-HIS-Cu-GO (PCG) nanocomposite sensor provides a unique platform for the fluorogenic detection of PPi having a very low limit of detection (LOD) of 0.60×10 M based on the strong affinity (1.0×10 M ) between the copper complex of PDI-HIS receptor and PPi. The intracellular detection of PPi using PCG also carried out in B16F10 cells where >10 times observed as compared to the PDI-HIS+Cu complex. Thus early cancer detection via PPi recognition in physiological conditions and in live cells was possible using PCG. Furthermore, the fabrication of PDI-HIS and PCG with PVA hydrogel films and on thin layer chromatography plates demonstrated the practical utility for the detection of PPi anions by 'off-on' response rapidly in a label free manner. |
| ISSN | 09565663 |
| Issue Number | Pt 1 |
| Volume Number | 89 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-03-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Diphosphates Analysis Graphite Chemistry Histidine Imides Nanostructures Perylene Analogs & Derivatives Animals Cell Line, Tumor Copper Melanoma Pathology Mice Ultrastructure Optical Imaging Methods Oxides Solubility Spectrometry, Fluorescence Water Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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