NDLI: Cloning and sequence analysis of cDNA for the luminescent protein aequorin.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sakaki, Y. Tsuji, F. I. Inouye, S. Iwanaga, S. Takagi, Y. Noguchi, M. Miyata, T.
Abstract	The luminescent jellyfish Aequorea contains a photoprotein, aequorin, which emits light by an intramolecular reaction in the presence of a trace amount of Ca2+. A cDNA library of Aequorea was constructed and clones carrying the cDNA for the Ca2+-dependent photoprotein were isolated by the method of colony hybridization using synthetic oligonucleotide probes. The primary structure of the protein deduced from the nucleotide sequence showed that the protein is composed of 189 amino acid residues and has three E-F hand structures that are characteristic for Ca2+-binding sites. The sequence also suggested that the protein has hydrophobic regions at which the protein may interact with its functional chromophore, coelenterazine.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	10
Volume Number	82
Language	English
Publisher	National Academy of Sciences
Publisher Date	1985-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Aequorin Genetics Luminescent Proteins Amino Acid Sequence Apoproteins Binding Sites Calcium Metabolism Cloning, Molecular DNA Luminescent Measurements Protein Conformation Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Cloning and sequence analysis of cDNA for the luminescent protein aequorin.