NDLI: The large form of hepatitis delta antigen is crucial for assembly of hepatitis delta virus.

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The large form of hepatitis delta antigen is crucial for assembly of hepatitis delta virus.

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Two-dimensional life?

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The large form of hepatitis delta antigen is crucial for assembly of hepatitis delta virus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chang, F. L. Wang, C. J. Chen, D. S. Tu, S. J. Chen, P. J.
Description	Author Affiliation: Chang FL ( Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei.);
Abstract	The virions of hepatitis delta virus (HDV) contain two species of HDV-specific protein, a large and a small form of hepatitis delta antigen (HDAg). We examined the role of individual HDAgs in virion assembly in cotransfection experiments. First, we constructed a replication-competent HDV mutant expressing only the small HDAg. When cotransfected with a plasmid expressing hepatitis B virus surface antigens to the HuH-7 cells, the mutant did not produce HDV virions, whereas the wild-type HDV clone did. Therefore, though the small HDAg is important for viral replication and is incorporated into the virus, the small-form delta antigen by itself is insufficient for virion formation. When the system was co-transfected with an additional plasmid providing the large HDAg, the HDV virion was then recovered. There was also evidence suggesting that the large HDAg could be copackaged into the HBsAg particles, without the presence of the HDV genome and the small HDAg. The results indicate a crucial role of the large HDAg in HDV assembly.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	19
Volume Number	88
Language	English
Publisher	National Academy of Sciences
Publisher Date	1991-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigens, Viral Physiology Hepatitis Delta Virus Growth & Development Cell Line Cloning, Molecular DNA Mutational Analysis Hepatitis B Surface Antigens Metabolism Immunology Ultrastructure Hepatitis Delta Antigens In Vitro Techniques Molecular Sequence Data Morphogenesis Oligonucleotides Chemistry RNA, Viral Structure-Activity Relationship Transfection Virion Virus Replication Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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