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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Broderius, M. Eide, D. Guerinot, M. L. Fett, J. |
| Description | Author Affiliation: Eide D ( Department of Biochemistry and Molecular Biology, University of Minnesota School of Medicine, Duluth, 55812, USA.); |
| Abstract | Iron is an essential nutrient for virtually all organisms. The IRT1 (iron-regulated transporter) gene of the plant Arabidopsis thaliana, encoding a probable Fe(II) transporter, was cloned by functional expression in a yeast strain defective for iron uptake. Yeast expressing IRT1 possess a novel Fe(II) uptake activity that is strongly inhibited by Cd. IRT1 is predicted to be an integral membrane protein with a metal-binding domain. Data base comparisons and Southern blot analysis indicated that IRT1 is a member of a gene family in Arabidopsis. Related sequences were also found in the genomes of rice, yeast, nematodes, and humans. In Arabidopsis, IRT1 is expressed in roots, is induced by iron deficiency, and has altered regulation in plant lines bearing mutations that affect the iron uptake system. These results provide the first molecular insight into iron transport by plants. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 11 |
| Volume Number | 93 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1996-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Arabidopsis Proteins Arabidopsis Metabolism Carrier Proteins Cation Transport Proteins Genes, Plant Multigene Family Plant Proteins Amino Acid Sequence Animals Genetics Growth & Development Biosynthesis Chemistry Cloning, Molecular Escherichia Coli Gene Expression Gene Library Kinetics Molecular Sequence Data Nematoda Oryza Sativa Plant Roots Protein Sorting Signals RNA, Messenger Recombinant Proteins Saccharomyces Cerevisiae Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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