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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Burns, M. A. Sammarco, T. S. Webster, J. R. Kaiser, A. R. Fields, Y. Johnsons, B. N. Foerster, B. Man, F. P. Burke, D. T. Mastrangelo, C. H. Jones, D. |
| Description | Author Affiliation: Burns MA ( Department of Chemical Engineering, University of Michigan, Ann Arbor 48109, USA.); |
| Abstract | Photolithographic micromachining of silicon is a candidate technology for the construction of high-throughput DNA analysis devices. However, the development of complex silicon microfabricated systems has been hindered in part by the lack of a simple, versatile pumping method for integrating individual components. Here we describe a surface-tension-based pump able to move discrete nanoliter drops through enclosed channels using only local heating. This thermocapillary pump can accurately mix, measure, and divide drops by simple electronic control. In addition, we have constructed thermal-cycling chambers, gel electrophoresis channels, and radiolabeled DNA detectors that are compatible with the fabrication of thermocapillary pump channels. Since all of the components are made by conventional photolithographic techniques, they can be assembled into more complex integrated systems. The combination of pump and components into self-contained miniaturized devices may provide significant improvements in DNA analysis speed, portability, and cost. The potential of microfabricated systems lies in the low unit cost of silicon-based construction and in the efficient sample handling afforded by component integration. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 11 |
| Volume Number | 93 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1996-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | DNA Genetics Polymerase Chain Reaction Instrumentation Automation Capillary Action DNA Primers Models, Genetic Molecular Sequence Data Restriction Mapping Silicon Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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