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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Steele, D. F. Fox, T. D. Butler, C. A. |
| Description | Author Affiliation: Steele DF ( Section of Genetics and Development, Cornell University, Ithaca, NY 14853-2703, USA.); |
| Abstract | Genetic code differences prevent expression of nuclear genes within Saccharomyces cerevisiae mitochondria. To bridge this gap a synthetic gene, ARG8m, designed to specify an arginine biosynthetic enzyme when expressed inside mitochondria, has been inserted into yeast mtDNA in place of the COX3 structural gene. This mitochondrial cox3::ARG8m gene fully complements a nuclear arg8 deletion at the level of cell growth, and it is dependent for expression upon nuclear genes that encode subunits of the COX3 mRNA-specific translational activator. Thus, cox3::ARG8m serves as a mitochondrial reporter gene. Measurement of cox3::ARG8m expression at the levels of steady-state protein and enzymatic activity reveals that glucose repression operates within mitochondria. The levels of this reporter vary among strains whose nuclear genotypes lead to under- and overexpression of translational activator subunits, in particular Pet494p, indicating that mRNA-specific translational activation is a rate-limiting step in this organellar system. Whereas the steady-state level of cox3::ARG8m mRNA was also glucose repressed in an otherwise wild-type strain, absence of translational activation led to essentially repressed mRNA levels even under derepressing growth conditions. Thus, the mRNA is stabilized by translational activation, and variation in its level may be largely due to modulation of translation. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 11 |
| Volume Number | 93 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1996-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Nucleus Metabolism DNA, Mitochondrial Electron Transport Complex IV Biosynthesis Gene Expression Regulation, Fungal Genes, Fungal Genes, Synthetic Membrane Proteins Protein Biosynthesis RNA, Messenger Saccharomyces Cerevisiae Genetics Transaminases Arginine DNA, Fungal Drug Effects Glucose Pharmacology Mitochondria Molecular Sequence Data RNA, Fungal Saccharomyces Cerevisiae Proteins Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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