NDLI: Reconstitution of HIV-1 rev nuclear export: independent requirements for nuclear import and export.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1998 Volume: 95

Year: 1998 Volume: 95 Issue: 26

Year: 1998 Volume: 95 Issue: 25

Year: 1998 Volume: 95 Issue: 24

Year: 1998 Volume: 95 Issue: 23

Year: 1998 Volume: 95 Issue: 22

Year: 1998 Volume: 95 Issue: 21

Year: 1998 Volume: 95 Issue: 20

Year: 1998 Volume: 95 Issue: 19

Year: 1998 Volume: 95 Issue: 18

Nitric oxide in plant immunity.

Skiing the black diamond slope: progress on the biochemistry of translesion DNA synthesis.

Diverse RNA substrates for aminoacylation: clues to origins?

The central questions in pain perception may be peripheral.

Ribonuclease A variants with potent cytotoxic activity.

A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator.

Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8 A resolution.

Protein kinase A-catalyzed phosphorylation of heat shock protein 60 chaperone regulates its attachment to histone 2B in the T lymphocyte plasma membrane

Resected RNA pseudoknots and their recognition by histidyl-tRNA synthetase.

Redesigning an FKBP-ligand interface to generate chemical dimerizers with novel specificity.

Zinc-dependent dimers observed in crystals of human endostatin.

Altering the anaerobic transcription factor FNR confers a hemolytic phenotype on Escherichia coli K12.

Substrate recruitment to cyclin-dependent kinase 2 by a multipurpose docking site on cyclin A.

Glucocorticoids and progestins signal the initiation and enhance the rate of myelin formation.

Design, synthesis, and characterization of a photoactivatable flavocytochrome molecular maquette.

Expression of mouse telomerase catalytic subunit in embryos and adult tissues.

Branch migration during Rad51-promoted strand exchange proceeds in either direction.

Cloning and characterization of a third human lysyl hydroxylase isoform.

Evolution of a quadripartite hybrid virus by interspecific exchange and recombination between replicase components of two related tripartite RNA viruses.

The three members of the pocket proteins family share the ability to repress E2F activity through recruitment of a histone deacetylase.

Farnesyltransferase inhibitors induce dramatic morphological changes of KNRK cells that are blocked by microtubule interfering agents.

Lagging strand replication of rolling-circle plasmids: specific recognition of the ssoA-type origins in different gram-positive bacteria.

The location of the carboxy-terminal region of γ chains in fibrinogen and fibrin D domains

A potential mediator of collagenous block copolymer gradients in mussel byssal threads.

A method for directed evolution and functional cloning of enzymes.

Multiple promoters direct the tissue-specific expression of novel N-terminal variant human vitamin D receptor gene transcripts.

Footprints on the viral DNA ends in moloney murine leukemia virus preintegration complexes reflect a specific association with integrase.

MEKK1/JNK signaling stabilizes and activates p53.

Implications of macromolecular crowding for signal transduction and metabolite channeling.

The domain structure of protoporphyrinogen oxidase, the molecular target of diphenyl ether-type herbicides.

Activation of iron regulatory protein-1 by oxidative stress in vitro

Structure of FokI has implications for DNA cleavage.

FokI dimerization is required for DNA cleavage.

Epidermal trans-urocanic acid and the UV-A-induced photoaging of the skin.

Homologous pairing in stretched supercoiled DNA.

The ATPase and protease domains of yeast mitochondrial Lon: roles in proteolysis and respiration-dependent growth.

Suppression of adenovirus E1A-induced apoptosis by mutated p53 is overcome by coexpression with Id proteins.

The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules.

WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: The proline glycine and methionine-rich motif

Reconstitution of HIV-1 rev nuclear export: independent requirements for nuclear import and export.

Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation.

Regulation of sorting and post-Golgi trafficking of rhodopsin by its C-terminal sequence QVS(A)PA

Inhibition of Stat1-mediated gene activation by PIAS1.

MLL, a mammalian trithorax-group gene, functions as a transcriptional maintenance factor in morphogenesis.

The Drosophila LIM-only gene, dLMO, is mutated in Beadex alleles and might represent an evolutionarily conserved function in appendage development.

The optx2 homeobox gene is expressed in early precursors of the eye and activates retina-specific genes.

Deletion of long-range regulatory elements upstream of SOX9 causes campomelic dysplasia.

DNase I-hypersensitive sites I and II of the human growth hormone locus control region are a major developmental activator of somatotrope gene expression.

Top-down versus bottom-up and the Ruritanian bean bug.

A conserved mode of head segmentation in arthropods revealed by the expression pattern of Hox genes in a spider.

Expression of homeobox genes shows chelicerate arthropods retain their deutocerebral segment.

Nonsynonymous substitution in abalone sperm fertilization genes exceeds substitution in introns and mitochondrial DNA.

Proposed mechanism for stability of proteins to evolutionary mutations.

Adult fitness consequences of sexual selection in Drosophila melanogaster.

Multiple independent origins of mitochondrial gene order in birds.

Replicational and transcriptional selection on codon usage in Borrelia burgdorferi.

Unusual horizontal transfer of a long interspersed nuclear element between distant vertebrate classes.

A general procedure for locating and analyzing protein-binding sequence motifs in nucleic acids.

Tn5/IS50 target recognition.

The APC variants I1307K and E1317Q are associated with colorectal tumors, but not always with a family history.

An SmtB-like repressor from Synechocystis PCC 6803 regulates a zinc exporter

Increased anxiety of mice lacking the serotonin1A receptor.

Two modes of transvection: enhancer action in trans and bypass of a chromatin insulator in cis.

New susceptibility locus for rheumatoid arthritis suggested by a genome-wide linkage study.

The reductive enzyme thioredoxin 1 acts as an oxidant when it is exported to the Escherichia coli periplasm.

PAGE-1, an X chromosome-linked GAGE-like gene that is expressed in normal and neoplastic prostate, testis, and uterus.

A cis-acting element that directs the activity of the murine methylation modifier locus Ssm1.

Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells.

Equilibrium distributions of microsatellite repeat length resulting from a balance between slippage events and point mutations.

A modifier screen in the eye reveals control genes for Krüppel activity in the Drosophila embryo.

A knob-associated tandem repeat in maize capable of forming fold-back DNA segments: are chromosome knobs megatransposons?

Determination of gene organization in individual haplotypes by analyzing single DNA fragments from single spermatozoa.

Identification of HLA class II-restricted determinants of Mycobacteriumtuberculosis-derived proteins by using HLA-transgenic, class II-deficient mice

beta-chemokines and neutralizing antibody titers correlate with sterilizing immunity generated in HIV-1 vaccinated macaques.

Antigenicity of fullerenes: antibodies specific for fullerenes and their characteristics.

Generation of mucosal cytotoxic T cells against soluble protein by tissue-specific environmental and costimulatory signals.

Endothelial cell death, angiogenesis, and microvascular function after castration in an androgen-dependent tumor: role of vascular endothelial growth factor.

A resistant genetic background leading to incomplete penetrance of intestinal neoplasia and reduced loss of heterozygosity in ApcMin/+ mice.

In vivo magnetic resonance vascular imaging using laser-polarized 3He microbubbles.

Synthesis and biological activities of potent peptidomimetics selective for somatostatin receptor subtype 2.

Conversion of a radioresistant phenotype to a more sensitive one by disabling erbB receptor signaling in human cancer cells.

Identification of inflections in T-cell counts among HIV-1-infected individuals and relationship with progression to clinical AIDS.

Functional expression of the Wilson disease protein reveals mislocalization and impaired copper-dependent trafficking of the common H1069Q mutation.

ETO, fusion partner in t(8;21) acute myeloid leukemia, represses transcription by interaction with the human N-CoR/mSin3/HDAC1 complex.

A viral gene that activates lytic cycle expression of Kaposi's sarcoma-associated herpesvirus.

Identification of the gene (lgtG) encoding the lipooligosaccharide beta chain synthesizing glucosyl transferase from Neisseria gonorrhoeae.

Segment-specific pattern of sympathetic preganglionic projections in the chicken embryo spinal cord is altered by retinoids.

Expression of a dominant negative TrkB receptor, T1, reveals a requirement for presynaptic signaling in BDNF-induced synaptic potentiation in cultured hippocampal neurons.

Characterization of a calmodulin kinase II inhibitor protein in brain.

Role for neuronally derived fractalkine in mediating interactions between neurons and CX3CR1-expressing microglia.

Mental chronometry using latency-resolved functional MRI.

Selectively enhanced contextual fear conditioning in mice lacking the transcriptional regulator CCAAT/enhancer binding protein delta.

Isoform-specific effects of human apolipoprotein E on brain function revealed in ApoE knockout mice: increased susceptibility of females.

beta-adrenergic receptor-induced activation of nerve growth factor gene transcription in rat cerebral cortex involves CCAAT/enhancer-binding protein delta.

Nerve growth factor rapidly suppresses basal, NMDA-evoked, and AMPA-evoked nitric oxide synthase activity in rat hippocampus in vivo.

How the owl resolves auditory coding ambiguity.

Modulation of a calcium-sensitive nonspecific cation channel by closely associated protein kinase and phosphatase activities.

A molecular mechanism for signaling between seven-transmembrane receptors: evidence for a redistribution of G proteins.

Nociceptin/orphanin FQ-induced nociceptive responses through substance P release from peripheral nerve endings in mice

Cyclooxygenase-2 inhibition prevents delayed death of CA1 hippocampal neurons following global ischemia

ZK200775: a phosphonate quinoxalinedione AMPA antagonist for neuroprotection in stroke and trauma.

Interaction between inducible nitric oxide synthase and cyclooxygenase-2 after cerebral ischemia.

Syntaxin 1A inhibits CFTR chloride channels by means of domain-specific protein-protein interactions.

The negative feedback actions of progesterone on gonadotropin-releasing hormone secretion are transduced by the classical progesterone receptor.

Two-dimensional confocal images of organization, density, and gating of focal $Ca^{2+}$ release sites in rat cardiac myocytes

Oocytes are a source of catecholamines in the primate ovary: evidence for a cell-cell regulatory loop.

Transport of uncharged organic solutes in Xenopus oocytes expressing red cell anion exchangers (AE1s).

In vivo responses of the primate corpus luteum to luteinizing hormone and chorionic gonadotropin.

A response regulator of cyanobacteria integrates diverse environmental signals and is critical for survival under extreme conditions.

A single gene of chloroplast origin codes for mitochondrial and chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana.

Protein kinase CK2 interacts with and phosphorylates the Arabidopsis circadian clock-associated 1 protein

Year: 1998 Volume: 95 Issue: 17

Year: 1998 Volume: 95 Issue: 16

Year: 1998 Volume: 95 Issue: 15

Year: 1998 Volume: 95 Issue: 14

Year: 1998 Volume: 95 Issue: 13

Year: 1998 Volume: 95 Issue: 12

Year: 1998 Volume: 95 Issue: 11

Year: 1998 Volume: 95 Issue: 10

Year: 1998 Volume: 95 Issue: 9

Year: 1998 Volume: 95 Issue: 8

Year: 1998 Volume: 95 Issue: 7

Year: 1998 Volume: 95 Issue: 6

Year: 1998 Volume: 95 Issue: 5

Year: 1998 Volume: 95 Issue: 4

Year: 1998 Volume: 95 Issue: 3

Year: 1998 Volume: 95 Issue: 2

Year: 1998 Volume: 95 Issue: 1

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Reconstitution of HIV-1 rev nuclear export: independent requirements for nuclear import and export.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Love, D. C. Sweitzer, T. D. Hanover, J. A.
Description	Author Affiliation: Love DC ( Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0851, USA.);
Abstract	The Rev protein of HIV-1 actively shuttles between nucleus and cytoplasm and mediates the export of unspliced retroviral RNAs. The localization of shuttling proteins such as Rev is controlled by the relative rates of nuclear import and export. To study nuclear export in isolation, we generated cell lines expressing a green fluorescent protein-labeled chimeric protein consisting of HIV-1 Rev and a hormone-inducible nuclear localization sequence. Steroid removal switches off import thus allowing direct visualization of the Rev export pathway in living cells. After digitonin permeabilization of these cells, we found that a functional nuclear export sequence (NES), ATP, and fractionated cytosol were sufficient for nuclear export in vitro. Nuclear pore-specific lectins and leptomycin B were potent export inhibitors. Nuclear export was not inhibited by antagonists of calcium metabolism that block nuclear import. These data further suggest that nuclear pores do not functionally close when luminal calcium stores are depleted. The distinct requirements for nuclear import and export argue that these competing processes may be regulated independently. This system should have wide applicability for the analysis of nuclear import and export.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	18
Volume Number	95
Language	English
Publisher	National Academy of Sciences
Publisher Date	1998-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cell Nucleus Metabolism Gene Products, Rev HIV-1 Adenosine Triphosphate Biological Transport Calcium HeLa Cells Hydrolysis Rev Gene Products, Human Immunodeficiency Virus Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in