NDLI: Histidine-tagged wild-type yeast actin: its properties and use in an approach for obtaining yeast actin mutants.

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Histidine-tagged wild-type yeast actin: its properties and use in an approach for obtaining yeast actin mutants.

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Histidine-tagged wild-type yeast actin: its properties and use in an approach for obtaining yeast actin mutants.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Karpova, T. Buzan, J. Frieden, C. Du, J.
Description	Author Affiliation: Buzan J ( Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA.);
Abstract	Wild-type and an N-terminal 6-histidine-tagged actin have each been expressed by using a yeast strain that contains the actin gene on a plasmid and not on the chromosome. Yeast strains have also been constructed that use two plasmids, one expressing the wild-type protein and the other the 6-histidine-tagged protein. Yeast cells can be grown with either plasmid alone or with both plasmids together and appear to be normal in that the growth rates of all the yeast strains are quite similar, as is the morphology of the yeast cells. The polymerization properties of the 6-histidine-tagged actin appear almost identical to wild-type actin expressed from the chromosome. When the wild-type and 6-histidine-tagged actin are coexpressed, they can be purified by standard techniques and then separated using nickel-nitrilotriacetate chromatography. The method can be used to prepare actin mutants including those that are nonfunctional or might not support yeast growth for other reasons.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	6
Volume Number	96
Language	English
Publisher	National Academy of Sciences
Publisher Date	1999-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Actins Genetics Metabolism Saccharomyces Cerevisiae Chemistry Dimerization Histidine Mutation Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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