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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Yang Miao, Jin Hu, Yiling Zhao, Xuejin Lou, Chunbo Bai, Chaoxian Xiang, Sihai Zhang, Lixin |
| Description | Author Affiliation: Bai C ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); Zhang Y ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); Zhao X ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); Hu Y ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); Xiang S ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); Miao J ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); Lou C ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); Zhang L ( Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology and Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China); |
| Abstract | There is a great demand for precisely quantitating the expression of genes of interest in synthetic and systems biotechnology as new and fascinating insights into the genetics of streptomycetes have come to light. Here, we developed, for the first time to our knowledge, a quantitative method based on flow cytometry and a superfolder green fluorescent protein (sfGFP) at single-cell resolution in Streptomyces. Single cells of filamentous bacteria were obtained by releasing the protoplasts from the mycelium, and the dead cells could be distinguished from the viable ones by propidium iodide (PI) staining. With this sophisticated quantitative method, some 200 native or synthetic promoters and 200 ribosomal binding sites (RBSs) were characterized in a high-throughput format. Furthermore, an insulator (RiboJ) was recruited to eliminate the interference between promoters and RBSs and improve the modularity of regulatory elements. Seven synthetic promoters with gradient strength were successfully applied in a proof-of-principle approach to activate and overproduce the cryptic lycopene in a predictable manner in Streptomyces avermitilis. Our work therefore presents a quantitative strategy and universal synthetic modular regulatory elements, which will facilitate the functional optimization of gene clusters and the drug discovery process in Streptomyces. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 39 |
| Volume Number | 112 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2015-09-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Biological Products Isolation & Purification Drug Discovery Flow Cytometry Green Fluorescent Proteins Metabolism Regulatory Elements, Transcriptional Genetics Streptomyces Chemistry Synthetic Biology Binding Sites Microscopy, Confocal Multigene Family Promoter Regions, Genetic Propidium Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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